The protective effect of Biling Weitong Granules on NSAIDs-induced small intestine injury
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摘要: 目的 研究荜铃胃痛颗粒在非甾体类抗炎药(nonsteroidal anti-inflammatory drugs，NSAIDs)相关小鼠小肠损伤中的作用及对小肠真菌组成及结构的影响。方法 将15只C57/BL6雄性小鼠随机分为对照组、模型组及荜铃胃痛颗粒干预组，每组各5只，采用10 mg/kg吲哚美辛诱导小鼠小肠黏膜损伤。对照组和模型组小鼠均予0.5%羧甲基纤维素钠灌胃，荜铃胃痛颗粒干预组予灌胃荜铃胃痛颗粒，连续3 d。第3天时，模型组和荜铃胃痛颗粒干预组采用吲哚美辛灌胃制备NSAIDs相关小鼠小肠损伤模型。取小鼠小肠组织，大体观察并进行苏木精-伊红染色，采用Reuter评分及Chiu氏6级评分方法进行小肠炎症损伤程度评分。采用18S rDNA测序技术检测各组小鼠小肠真菌组成及结构改变。结果 吲哚美辛诱导小鼠小肠黏膜明显损伤，Reuter评分及组织学评分升高(P＜0.05)，肠道真菌α多样性和β多样性下降，而给予荜铃胃痛颗粒干预后小鼠黏膜损伤有所恢复，Reuter评分及组织学评分下降，真菌α多样性和β多样性升高(P＜0.05)。FunGuide功能分析显示模型组中动物病原菌、未定义腐生真菌及木质腐生真菌等比例明显减少，而荜铃胃痛颗粒干预后上述功能菌属有所恢复。结论 荜铃胃痛颗粒能够缓解NSAIDs相关的小鼠小肠损伤，并影响肠道真菌组成及结构。Abstract: Objective To explore the protective effect of Biling Weitong Granules(BLWTGs) on the nonsteroidal anti-inflammatory drugs-related small intestine injury in mice and its regulatory effect on the composition of small intestine fungi.Methods Fifteen C57/BL6 male mice were randomly divided into the control group, model group and BLWTGs-treated group(5 animals/group). 10 mg/kg indomethacin was used to induce intestinal mucosal injury in mice. The control and model groups were gavaged with 0.5% sodium carboxymethylcellulose, and the BLWTGs-treated group were gavaged with BLWTGs for 3 consecutive days. On the 3rd day, the model group and BLWTGs-treated groups were administrated with indomethacin to induce NSAIDs-related small intestinal injury in mice. The small intestinal tissues of mice observed grossly and stained with H&E, and the degree of small intestinal injury was scored using Reuter score and Chiu's 6-grade scoring method, respectively. 18S rDNA technology was performed to examine the composition of small intestine fungi in mice.Results Mice in the model group showed small intestinal mucosal rupture, elevated gross index and histologic scores(P < 0.05), and the composition of intestine fungi showed decreased intestinal fungal α-diversity and β-diversity. Whereas BLWTGs significantly attenuated indomethacin-induced small intestine injury, with downregulated of gross index and histologic score(P < 0.05). Additionally, the α-diversity and β-diversity of small intestine fungi were increased. FunGuide analysis revealed that the proportions of animal pathogens, undefined saprophytic fungi and xylideous saprophytic fungi in the model group were significantly reduced, while the above-mentioned functional bacteria were restored after the intervention of Biling Weitong Granules.Conclusion Biling Weitong Granules alleviated NSAIDs-related small intestinal injury in mice and affect intestinal fungal composition and structure.
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