Exploring the changes of mucosal protein expression in intestinal metaplasia patients based on gastric "inflammatory-carcinoma" transformation
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摘要: 目的 基于胃“炎-癌”转化探析肠上皮化生患者黏膜蛋白表达变化。方法 选取非萎缩性胃炎、慢性萎缩性胃炎、肠上皮化生患者各10例，免疫组织化学法检测溴素蛋白4(olfactomedin 4，OLFM4)、尾型同源盒因子2(caudal type homeobox transcription factor 2，CDX2)、Krüppel样转录因子4(Krüppel-like factor 4，KLF4)、肝细胞核因子4α(hepatocyte nuclear factor 4α，HNF4α)、共同急性淋巴母细胞性白血病抗原10(CB-CALLA，CD10)等蛋白的表达情况。结果 OLFM4在非萎缩性胃炎及慢性萎缩性胃炎胃黏膜中散在表达，在肠上皮化生中弥漫性表达；CDX2在非萎缩性胃炎及慢性萎缩性胃炎胃黏膜上皮中几乎不表达，在肠上皮化生中呈中度阳性表达；KLF4在非萎缩性胃炎和慢性萎缩性胃炎中仅在胃腔侧上皮细胞核呈中度阳性表达，在基底侧阴性表达，在肠上皮化生中在胃腔侧上皮细胞核中呈强阳性表达，在基底侧细胞核中呈中度阳性表达；HNF4α在非萎缩性胃炎和慢性萎缩性胃炎中呈阴性表达，在肠上皮化生中在胃腔侧呈现弥漫性中度阳性表达；CD10在非萎缩性胃炎和慢性萎缩性胃炎中呈中度阳性弥漫性表达，在肠上皮化生中呈强阳性弥漫性表达。结论 OLFM4、CDX2、KLF4、HNF4α、CD10可作为胃“炎-癌”转化肠上皮化生特异性标志物。
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关键词:
- 胃"炎-癌"转化 /
- 肠上皮化生 /
- 临床样本 /
- 免疫组织化学 /
- 溴素蛋白4 /
- 尾型同源盒因子2 /
- Krüppel样转录因子4 /
- 肝细胞核因子4α /
- 共同急性淋巴母细胞性白血病抗原10
Abstract: Objective Exploring the changes of mucosal protein expression in intestinal metaplasia patients based on gastric "inflammatory-carcinoma" transformation.Methods Patients with non-astrophic gastritis, chronic atrophic gastritis and intestinal metaplasia each ten were selected to detect the expression of olfactomedin 4(OLFM4), caudal type homeobox transcription factor 2(CDX2), Krüppel-like factor 4(KLF4), hepatocyte nuclear factor 4α(HNF4α), CB-CALLA(CD10) and other proteins by immunohistochemistry.Results OLFM4 is expressed scattered in non-astrophic gastritis and chronic atrophic gastritis gastric mucosa, and is expressed diffusely in intestinal metaplasia. CDX2 is almost not expressed in non-astrophic gastritis and chronic atrophic gastritis, but is moderately positive in intestinal metaplasia. The expression of KLF4 in non-astrophic gastritis and chronic atrophic gastritis was only moderately positive in the cell nucleus of the gastric lumen epithelium and negative in the basal side. In intestinal metaplasia, KLF4 is showed strong positive expression in the nucleus of the gastric lumen epithelium and moderate positive expression in the nucleus of the basal side. HNF4α is negatively expressed in non-astrophic gastritis and chronic atrophic gastritis. In intestinal metaplasia, diffuse moderately positive expression of HNF4α is found in the gastric lumen epithelium. CD10 is diffuse moderately positive in non-astrophic gastritis and chronic atrophic gastritis and diffuse strongly positive in intestinal metaplasia.Conclusion OLFM4, CDX2, KLF4, HNF4α and CD10 can be used as specific intestinal metaplasia markers of gastric "inflammatory-carcinoma" transformation. -
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