胆宁片治疗豚鼠胆色素结石的实验研究

刘莹露; 赵航; 陈萍; 徐天舒

doi:10.3969/j.issn.1671-038X.2014.10.05

胆宁片治疗豚鼠胆色素结石的实验研究

- 南京大学医学院附属鼓楼医院中医科, 南京 210008
基金项目:
中国中西医结合学会—和黄科研基金项目（No：2012004）

详细信息

作者简介:
刘莹露,女,住院医师,硕士,研究方向中医内科内分泌代谢

通讯作者: 徐天舒,E-mail:tianshuxu2007@126.com

中图分类号: R285.5

收稿日期: 2014-06-29

The experimental study of Danning Tablet on guinea pigs with bile pigment calculus

- Department of Traditional Chinese Medicine, Nanjing Drum Tower Hospital Affiliated to Medical School of Nanjing University, Nanjing 210008, China

More Information

Corresponding author: XU Tian-shu, E-mail: tianshuxu2007@126.com

Received Date: 29 June 2014

摘要

摘要: [目的]研究胆宁片治疗豚鼠胆色素结石的作用及机制。[方法]构建豚鼠胆色素结石模型，给予胆宁片小、中、大剂量治疗，治疗后观察豚鼠成石率，并用ELISA法测定血清中IL-15、IL-1β及胆汁中IL-15含量。[结果]胆宁片中、大剂量治疗后的豚鼠成石率较模型组下降，差异有统计学意义（P<0.01）；与模型组比较，胆宁片各剂量组血清中IL-15、IL-1β及胆汁中IL-15含量减少，差异有统计学意义（P<0.01），且胆宁片各剂量组间比较差异也有统计学意义（P<0.01）。[结论]胆宁片能有效治疗豚鼠胆色素结石，其机制与减少IL-15、IL-1β含量有关。
- 胆宁片 /
- 胆色素结石 /
- 白细胞介素-15 /
- 白细胞介素-1β
Abstract: [Objective] To explore the therapeutic effect and the mechanism of Danning Tablet on guinea pigs with bile pigment calculus.[Methods] We established the model of guinea pigs with bile pigment calculus and used Danning Tablets of low, medium and high doses to cure them.After treatment, the rate of gallstone and the content of IL-15 and IL-1β in the serum as well as the content of IL-15 in the bill were measured with enzyme-linked immunosorbent assay(ELISA) method.[Results] Compared with the model group, the rate of gallstone in medium and high dose Danning Tablet groups was lower than that of model group(P<0.01);Compared with the model group, the content of IL-15 in the serum and bile as well as the content of IL-1β in the serum were significantly decreased, and there was significant difference(P<0.01).There was also significant difference among three-dose groups(P<0.01).[Conclusion] Danning Tablet has the effects of treating guinea pigs with bile pigment calculus.The mechanism is related to the decrease of the content of IL-15 and IL-1β.
- Danning Tablet /
- bile pigment calculus /
- IL-15 /
- IL-1β

HTML全文

参考文献(10)

[1]	朱培庭, 张静哲, 王以实, 等.胆宁片、胆通、熊去氧胆酸治疗慢性胆道感染、胆石病的临床疗效对照研究[J].中国中西医结合外科杂志, 1995, 1(4):205-209.
[2]	向阳, 项建斌, 马保金, 等.胆宁片对胆石症患者胆汁成分和成石趋势的影响[J].上海医学, 2008, 31(2):104-106.
[3]	程相超, 赵建红, 袁启东, 等.胆宁片在胆道结石治疗中的疗效观察[J].中国社区医师, 2013, 15(7):215-216.
[4]	李坤, 邓兆斌, 侯宪琴, 等.胆宁片治疗慢性胆囊炎及胆石症的体会[J].现代中西医结合杂志, 2011, 20(10):1228-1228.
[5]	张静喆, 梁晓强, 章学林, 等.清胆胶囊与养肝利胆颗粒对豚鼠胆色素结石炎症反应环节的影响[J].上海中医药大学学报, 2009, 23(5):51-54.
[6]	FEHNIGER T A, CALIGIURI M A.Interleukin 15:biology and relevance to human disease[J].Blood, 2001, 97(1):14-32.
[7]	BADOLATO R, PONZI A N, MILLESIMO M, et al.Interleukin-15(IL-15) induces IL-8and monocyte chemotatic protein 1production in human monocytes[J].Blood, 1997, 90(7):2804-2809.
[8]	王玉春, 李涛, 冯丽.IL-15在豚鼠胆囊炎形成中的作用[J].医学研究杂志, 2009, 28(3):46-47.
[9]	吴晓岚, 赵书芬.IL-1β致热机制的研究进展[J].解剖科学进展, 2004, 10(3):267-270.
[10]	王玉春, 苏富琴, 苗术, 等.金茵颗粒剂抗豚鼠胆囊炎的实验研究[J].医学研究杂志, 2008, 37(9):90-91.