沙利度胺对溃疡性结肠炎大鼠结肠组织MMP-9、COX-2、IL-8水平的影响

段春兰; 宁宝森; 唐莉

doi:10.3969/j.issn.1671-038X.2014.10.04

沙利度胺对溃疡性结肠炎大鼠结肠组织MMP-9、COX-2、IL-8水平的影响

- 内蒙古包头医学院第一附属医院消化科, 内蒙古包头 014010
基金项目:
内蒙古包头市卫生基金项目（No：Wsjj2013081）

详细信息

作者简介:
段春兰,女,副主任医师、副教授,主要从事消化系统疾病的诊断及治疗研究

通讯作者: 段春兰,E-mail:dcl135@163.com

中图分类号: R574.62

收稿日期: 2014-07-02

Effect of thalidomide on the expression of MMP-9,COX-2 and IL-8 in rat model of ulcerative colitis

- Department of Gastroenterology, the First Affiliated Hospital of Baotou Medical College, Inner Mongolia Baotou 014010, China

More Information

Corresponding author: DUAN Chun-lan, E-mail: dcl135@163.com

Received Date: 02 July 2014

摘要

摘要: [目的]探讨沙利度胺治疗溃疡性结肠炎大鼠的疗效及对结肠组织中基质金属蛋白酶-9（MMP-9）、环氧合酶-2（COX-2）、白介素-8（IL-8）表达水平的影响。[方法]将40只Wistar大鼠随机分为空白对照组、模型组、柳氮磺胺吡啶（SASP）组、沙利度胺组，每组10只。应用2，4，6三硝基苯磺酸与乙醇的混合物复制溃疡性结肠炎大鼠模型，除空白对照组和模型组正常饲养不进行药物治疗外，柳氮磺胺吡啶组给予SASP混悬液100 mg/（kg·d）灌胃，沙利度胺组给予沙利度胺片混悬液200 mg/（kg·d）灌胃。14 d后处死全部大鼠，观察大鼠结肠组织大体形态、结肠炎症活动指数（DAI）评分和病理学的改变，并采用免疫组织化学法检测结肠组织中MMP-9、COX-2、IL-8的表达水平。[结果]沙利度胺能显著降低溃疡性结肠炎大鼠DAI评分和结肠组织中MMP-9、COX-2、IL-8的表达水平，并对损伤黏膜有较好的修复作用。[结论]沙利度胺对溃疡性结肠炎大鼠有较好的疗效，主要表现在促进黏膜愈合、降低组织中MMP-9、COX-2、IL-8的表达水平等方面。
- 沙利度胺 /
- 溃疡性结肠炎 /
- 基质金属蛋白酶-9 /
- 环氧合酶-2 /
- 白介素-8
Abstract: [Objective] To explore the efficacy of thalidomide in rat model of ulcerative colitis and the expression of MMP-9, COX-2 and IL-8 in colon mucosa.[Methods] Forty Wistar rats were randomly divided into control group, model group, sulfasalazine group and thalidomide group, every group containing 10 rats.TNBS and alcohol mixture were used to build the model of ulcerative colitis.Rats of sulfasalazine group were given orally 100 mg/(kg·d).Rats of thalidomide group were given orally 200 mg/(kg·d).The rats of control group and model group were fed with normal feeding without any drug.All rats were killed after 14 days. The general morphology of colon tissue, DAI score and pathology changes were observed.The expression of MMP-9, COX-2 and IL-8 in colon mucosa was detected by immunohistochemistry.[Results] Thalidomide significantly reduced the DAI score and the expression of MMP-9, COX-2 and IL-8 in colon mucosa.Simultaneously, it played a role in repairing mucosal injury.[Conclusion] Thalidomide has a good effect on rat model of ulcerative colitis, mainly in promoting mucosal healing and reducing the expression of MMP-9, COX-2 and IL-8 in colon mucosa.
- thalidomide /
- ulcerative colitis /
- MMP-9 /
- COX-2 /
- IL-8

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参考文献(10)

[1]	王玉芳, 欧阳钦, 胡仁伟, 等.炎症性肠病流行病学研究进展[J].胃肠病学, 2013, 18(1):48-51.
[2]	陈曦, 田耀州, 夏军权.溃疡性结肠炎治疗研究进展[J].南京中医药大学学报, 2013, 29(3):293-296.
[3]	张伟, 王建莉.炎症性肠病的致病机理和治疗研究进展[J].国际免疫学杂志, 2009, 32(2):121-126.
[4]	鲁兵, 余万桂, 吴国栋.溃疡性结肠炎模型建立的研究进展[J].长江大学学报(自科版)医学卷, 2013, 10(2):102-105.
[5]	郑秀霞.沙利度胺对葡聚糖硫酸钠诱导的大鼠溃疡性结肠炎疗效观察[D].福建:福建医科大学, 2011.
[6]	王烜, 欧阳钦.沙利度胺对炎症性肠病疗效的实验研究[J].四川大学学报(医学版), 2005, 36(4):548-551.
[7]	MURANO M, MAEMURA K, HIRATA I, et al.Therapeutic effect of intracolonically administered nuclear factor kappa B (p65) antisense oligonucleotide on mouse dextran sulphate sodium (DSS) -induced colitis[J].Clin Exp Immunol, 2000, 120(1):51-58.
[8]	李敏丽, 瞿勇, 缪应雷.炎症性肠病与精神心理因素相关性研究[J].昆明医科大学学报, 2013, 34(2):32-38.
[9]	刘青梅, 韩辅.肠康颗粒对溃疡性结肠炎大鼠结肠组织MMP-3、MMP-9水平的影响[J].中国老年学杂志, 2012, 32(9):1886-1887.
[10]	PAIOTTI A P, ARTIGIANI NETO R, FORONES N M, et al.Immunoexpression of cyclooxygenase-1and-2in ulcerative colitis[J].Braz J Med Biol Res, 2007, 40(7):911-918.