藤龙补中汤对大肠癌RKO细胞转移相关基因表达影响

胡兵; 安红梅; 郑佳露; 闫霞; 黄晓伟; 李淼

doi:10.3969/j.issn.1671-038X.2018.05.07

藤龙补中汤对大肠癌RKO细胞转移相关基因表达影响

- 1. 上海中医药大学附属龙华医院中医肿瘤研究所肿瘤科, 上海 200032;
- 2. 上海中医药大学附属龙华医院科技处, 上海 200032
基金项目:
国家自然科学基金(No:81473625)

上海市科技支撑项目(No:16401902500)

龙华医院科技创新项目(No:CX201756)

详细信息

通讯作者: 胡兵,E-mail:beearhu@hotmail.com

中图分类号: R735.3

收稿日期: 2018-01-10

Effects of Teng-Long-Bu-Zhong-Tang on metastasis related genes expression in colorectal carcinoma RKO cells

- 1. Institute of Traditional Chinese Medicine in Oncology, Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China;
- 2. Department of Science&Technology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China

More Information

Corresponding author: HU Bing, E-mail: beearhu@hotmail.com

Received Date: 10 January 2018

摘要

摘要: [目的]观察藤龙补中汤对人大肠癌RKO细胞转移相关基因表达影响。[方法]藤龙补中汤处理RKO细胞,TRIzol试剂提取RNA,试剂盒纯化RNA,肿瘤转移PCR芯片(Tumor metastasis PCR array)检测基因表达。[结果]藤龙补中汤作用RKO细胞后,CD82、CDH1、CDKN2a、KISS1、SSTR2和TIMP2表达升高,FLT4、CD44、FGFR4、ITGB3、MMP3、MMP9、SMAD2和TGFB1表达降低,涉及细胞粘附、移动、侵袭、基质重构、上皮-间质转化和淋巴管生成等与转移相关的生物过程,CD44还与大肠癌干细胞和循环大肠癌细胞相关。[结论]藤龙补中汤可调控RKO细胞多个转移相关基因表达,并可能对上皮-间质转化、大肠癌干细胞、循环大肠癌细胞和淋巴管生成有抑制作用。
- 大肠癌 /
- 中医药 /
- 藤龙补中汤 /
- 转移 /
- 基因表达
Abstract: [Objective]To observe the effect of Teng-Long-Bu-Zhong-Tang(TLBZT)on metastasis related genes expression in human colorectal carcinoma RKO cells.[Methods]RKO cells were treated with TLBZT.RNA were extracted by TRIzol regent and purified with commercial kit.Gene expressions were detected by tumor metastasis PCR array.[Results]After TLBZT treatment,CD82,CDH1,CDKN2a,KISS1,SSTR2 and TIMP2 were up-regulated,FLT4,CD44,FGFR4,ITGB3,MMP3,MMP9,SMAD2 and TGFB1 were down-regulated in RKO cells.These genes involve metastasis related bioprocesses,such as cell adhesion,migration,invasion,extracellular matrix (ECM)remodeling,epithelial-mesenchymal transition(EMT)and lymphangiogenesis.CD44 also related to colorectal cancer stem cells and circulating colorectal cancer cells.[Conclusion]TLBZT regulates multiple metastasis related gens expression in RKO cells,and may inhibit EMT,colorectal cancer stem cells and circulating colorectal cancer cells.
- Colorectal cancer /
- Traditional Chinese Medicine /
- Teng-Long-Bu-Zhong-Tang /
- Metstasis /
- Gene expression

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