Effect of electroacupuncture combined with Tuina at Shenque acupoint on gut microbiota in patients with functional constipation
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摘要: 目的 探讨神阙穴电针联合推拿对功能性便秘患者肠道菌群的影响。方法 选取于西南医科大学附属中医医院就诊的功能性便秘患者80例，采用随机分组原则分为对照组、神阙穴电针组、推拿组、神阙穴电针联合推拿组，每组20例。对照组不给予治疗，神阙穴电针组给予电针神阙穴治疗，推拿组给予腹部推拿治疗，神阙穴电针联合推拿组给予电针神阙穴及腹部推拿治疗，对比治疗后临床疗效，并采集粪便样本进行16S rDNA基因测序。结果 经过治疗后，神阙穴电针联合推拿组总有效率、大便性状改善率明显较高(P< 0.05)；肠道菌群微生物中，对照组患者罗氏菌属(Roseburia)、拟杆菌(Bacteroides)丰度较高，神阙穴电针联合推拿组患者罗氏菌属(Roseburia)、拟杆菌(Bacteroides)、巨单胞菌属(Megamonas)、粪杆菌属(Faecalibacterium)、布劳特氏菌属(Blautia)相对丰度增加。结论 采用神阙穴电针联合推拿治疗功能性便秘患者具有较好的治疗效果，并具有调控患者肠道微生物菌群结构的作用。Abstract: Objective To investigate the effect of Shenque acupoint acupuncture combined with Tuina on the gut microbiota of patients with functional constipation.Methods Eighty patients with functional constipation attending our hospital were selected and divided into control group, Shenque acupoint acupuncture group, Tuina group and Shenque acupoint acupuncture combined with Tuina group with 20 cases in each group using the randomized grouping principle. The control group was not given treatment, the Shenque acupoint acupuncture group was given electroacupuncture of Shenque acupoints, the Tuina group was given abdominal Tuina treatment, and the Shenque acupoint acupuncture combined with Tuina group was given electroacupuncture of Shenque acupoints and abdominal Tuina treatment, and the clinical efficacy was compared after treatment, and stool samples were collected for 16S rDNA gene sequencing.Results After treatment, the total phase rate and stool trait improvement rate were significantly higher in the Shenque acupoint acupuncture combined with Tuina group(P< 0.05); Among the gut microbiota, the abundances ofRoseburiaandBacteroideswere higher in the control group, and the relative abundances ofRoseburia,Bacteroides,Megamonas,FaecalibacteriumandBlautiawere increased in the Shenque acupoint acupuncture combined with Tuina group.Conclusion The treatment of patients with functional constipation using Shenque acupoint electroacupuncture combined with Tuina has a good therapeutic effect and has the effect of regulating the structure of the gut microbiota of patients.
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Key words:
- functional constipation /
- electroacupuncture /
- Tuina /
- Shenque acupoint /
- gut microbiota
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表 1 各组研究对象基本资料比较
X±S 组别 例数 性别/例 平均年龄/岁 平均病程/年 男 女 对照组 20 8 12 52.70±12.71 10.85±5.69 神阙穴电针组 20 10 10 53.70±15.08 11.80±6.94 推拿组 20 7 13 54.25±12.10 12.10±5.80 神阙穴电针+推拿组 20 9 11 48.40±9.54 9.54±5.62 
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表 2 各组临床疗效分析
例 组别 例数 疗效 总有效率/% P 治愈 显效 有效 无效 对照组 20 神阙穴电针组 20 2 9 6 3 85.0 推拿组 20 2 8 6 4 80.0 0.020 神阙穴电针+推拿组 20 3 11 4 2 90.0
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表 3 各组患者大便性状改善率比较
例 组别 例数 大便转正常例数 非正常大便例数 改善率/% 对照组 20 神阙穴电针组 20 15 5 75.0 推拿组 20 14 6 70.0 神阙穴电针+推拿组 20 17 3 85.01) 与推拿组比较，1)P < 0.05。
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