食管鳞状细胞癌患者组织中RAB1A基因表达的相关研究及其临床意义

梁晓虎; 田海卫

doi:10.3969/j.issn.1671-038X.2019.08.02

食管鳞状细胞癌患者组织中RAB1A基因表达的相关研究及其临床意义

梁晓虎,
田海卫^,

- 宝鸡市人民医院胸心外科, 陕西宝鸡 721000

详细信息

作者简介:
梁晓虎,男,本科,主治医师,研究方向:肿瘤

通讯作者: 田海卫,E-mail:496854906@qq.com

中图分类号: R735.1

收稿日期: 2019-04-24

The expression pattern and clinic value of RAB1A in esophageal squamous cell carcinoma

LIANG Xiao-hu,
TIAN Hai-wei^,

- Department of Cardiothoracic Surgery, Baoji People's Hospital, Baoji 721000, China

More Information

Corresponding author: TIAN Hai-wei, E-mail: 496854906@qq.com

Received Date: 24 April 2019

摘要

摘要: [目的]探讨RAB1A在食管鳞状细胞癌患者中的表达及其临床意义。[方法]采用实时荧光定量PCR检测课题组2013~2015年收集的126例确诊食管鳞状细胞癌患者的癌组织及对应癌旁正常组织中RAB1A的表达量,并根据其表达量高低分为高表达组和低表达组;分析RAB1A表达量与食管鳞状细胞癌患者临床特征、生存期之间的关系;通过过表达RAB1A的表达后检测其对食管鳞状细胞癌细胞系增殖、迁移和侵袭能力的影响。[结果]RAB1A在食管鳞状细胞癌组织中表达量高于对应癌旁正常组织(0.0113±0.0194 vs.0.0071±0.0098,P=0.0123);RAB1A高表达与食管鳞状细胞癌患者家族肿瘤史(P=0.002)、饮酒(P=0.005)、体质指数(P=0.001)、分期(P=0.024)、淋巴结转移(P＜0.001)、远端转移(P＜0.001)及分化程度(P＜0.001)相关;RAB1A高表达与食管鳞状细胞癌患者生存期明显缩短(P＜0.001)。RAB1A过表达后促进食管鳞状细胞癌细胞增殖(F=151.9,P＜0.001)、transwell迁移(100.4±14.2 vs.51.2±10.6,P=0.009)、划痕实验迁移距离[(655.0±43.9)μm vs.(320.7±115.2) μm,P=0.0003]和transwell侵袭(79.2±13.4 vs.46.2±17.6,P=0.0014)。[结论]RAB1A在食管鳞状细胞癌中高表达,且高表达将缩短食管鳞状细胞癌患者生存期,过表达RAB1A能够促进食管鳞状细胞癌细胞增殖、迁移和侵袭能力。
- 食管鳞状细胞癌 /
- RAB1A /
- 预后
Abstract: [Objective]To detect the expression and clinical value of RAB1A in patients with esophageal squamous cell carcinoma(ESCC).[Methods]Real-time quantitative PCR(RT-PCR)was used to detect the expression level of RAB1A in cancer tissues and adjacent normal tissues of 126 patients diagnosed with ESCC which were collected from 2013 to 2015 by the research group,the expression level was divided into high expression group and low expression group according to the expression level.The relationship between RAB1A expression and clinical characteristics and survival of patients with ESCC were analyzed,the effect of RAB1A overexpression on the proliferation,migration and invasion ability of esophageal squamous cell carcinoma lines was detected.[Results]RAB1A was upregulated in ESCC cancer tissues than that in adjacent normal tissues(0.0113±0.0194 vs.0.0071±0.0098,P=0.0123),and its high expression was correlated with the family history of cancer(P=0.002),alcohol drinking(P=0.005),BMI(P=0.001),clinical stage(P=0.024),lymph node metastasis(P＜0.001),distal metastasis(P＜0.001),and pathologic type(P＜0.001).The patients with a higher RAB1A expression level had a shorter overall survival rate(P＜0.001).Over expression of RAB1A promotes proliferation of ESCC cell lines(F=151.9,P＜0.001),and promotes ESCC cell lines migration(100.4±14.2 vs.51.2±10.6,P=0.009)in trans-well assay,(655.0±43.9)μm vs.(320.7±115.2)μm,P=0.0003)in wound healing assay,and invasion(79.2±13.4 vs.46.2±17.6,P=0.0014).[Conclusion]RAB1A was highly expressed in ESCC,higher expression of RAB1A shorts the overall survival rate of ESCC patients.Upregulate the expression level of RAB1A promotes ESCC cell lines proliferation,migration and invasion.
- esophageal squamous cell carcinoma /
- RAB1A /
- survival

HTML全文

参考文献(24)

[1]	Siegel R L, Miller K D, Jemal A.Cancer statistics, 2018[J].CA Cancer J Clin, 2018, 68 (1):7-30.
[2]	Allemani C, Matsuda T, Carlo V D, et al.Global surveillance of trends in cancer survival 2000-14 (CONCORD-3):analysis of individual records for37513025patients diagnosed with one of 18cancers from 322population-based registries in 71 countries[J].The Lancet, 2018, 391 (10125):1023-1075.
[3]	Nj K, Ab V, Ms C, et al.Global, regional, and national levels and causes of maternal mortality during 1990-2013:a systematic analysis for the Global Burden of Disease Study 2013[J].Lancet, 2014, 384 (9947):980-1004.
[4]	Zeng H, Zheng R, Zhang S, et al.Esophageal cancer statistics in China, 2011:Estimates based on 177cancer registries[J].Thorac Cancer, 2016, 7 (2):232-237.
[5]	杜灵彬, 魏文强.中国人群食管鳞癌生存分析研究新进展[J].实用肿瘤学杂志, 2018, 32 (4):344-348.
[6]	郑荣寿, 张思维, 吴良有, 等.中国肿瘤登记地区2008年恶性肿瘤发病和死亡分析[J].中国肿瘤, 2012, 21 (1):1-8.
[7]	Zheng R, Zeng H, Zhang S, et al.Estimates of cancer incidence and mortality in China, 2013[J].Chin J Cancer, 2017, 36 (1):66.
[8]	曾红梅, 郑荣寿, 张思维, 等.1989-2008年中国恶性肿瘤死亡趋势分析[J].中华肿瘤杂志, 2012, 34 (7):525-531.
[9]	Islami F, Chen W, Yu X Q, et al.Cancer deaths and cases attributable to lifestyle factors and infections in China, 2013[J].Ann Oncol, 2017, 28 (10):2567-2574.
[10]	Nikoshkov A, Broliden K, Attarha S, et al.Expression pattern of the PRDX2, RAB1A, RAB1B, RAB5Aand RAB25genes in normal and cancer cervical tissues[J].Int J Oncol, 2015, 46 (1):107-112.
[11]	Tanaka M, Mun S, Harada A, et al.Hsc70contributes to cancer cell survival by preventing Rab1Adegradation under stress conditions[J].PLoS one, 2014, 9 (5):e96785.
[12]	Sanchez-gurmaches J, Guertin D A.mTORC1gRABs the Golgi[J].Cancer Cell, 2014, 26 (5):601-603.
[13]	Thomas J D, Zhang Y J, Wei Y H, et al.Rab1Ais an mTORC1activator and a colorectal oncogene[J].Cancer Cell, 2014, 26 (5):754-769.
[14]	Sun T, Wang X, He H H, et al.MiR-221promotes the development of androgen independence in prostate cancer cells via downregulation of HECTD2 and RAB1A[J].Oncogene, 2014, 33 (21):2790-800.
[15]	Abd Elmageed Z Y, Yang Y, Thomas R, et al.Neoplastic reprogramming of patient-derived adipose stem cells by prostate cancer cell-associated exosomes[J].Stem Cells, 2014, 32 (4):983-997.
[16]	Bray F, Ferlay J, Soerjomataram I, et al.Global cancer statistics 2018:GLOBOCAN estimates of incidence and mortality worldwide for 36cancers in 185countries[J].CA Cancer J Clin, 2018, 68 (6):394-424.
[17]	Torre L A, Bray F, Siegel R L, et al.Global cancer statistics, 2012[J].CA Cancer J Clin, 2015, 65 (2):87-108.
[18]	Chen W, Zheng R, Baade P D, et al.Cancer statistics in China, 2015[J].CA Cancer J Clin, 2016, 66 (2):115-132.
[19]	Chen W.Cancer statistics:updated cancer burden in China[J].Chin J Cancer Res, 2015, 27 (1):1.
[20]	Li W, Zheng J, Deng J, et al.Increased levels of the long intergenic non-protein coding RNA POU3F3promote DNA methylation in esophageal squamous cell carcinoma cells[J].Gastroenterology, 2014, 146 (7):1714-1726, e5.
[21]	Park J S, Heo J S, Chang H S, et al.Association analysis of member RAS oncogene family gene polymorphisms with aspirin intolerance in asthmatic patients[J].DNA Cell Biol, 2014, 33 (3):155-161.
[22]	Shimada K, Uzawa K, Kato M, et al.Aberrant expression of RAB1Ain human tongue cancer[J].Br J Cancer, 2005, 92 (10):1915-1921.
[23]	Ishida M, Ohbayashi N, Fukuda M.Rab1 Aregulates anterograde melanosome transport by recruiting kinesin-1to melanosomes through interaction with SKIP[J].Sci Rep, 2015, 5 (8):238.
[24]	Ruffell B, Chang-shtrachan D, Chan V, et al.RAB1A Promotes Oncogenesis in Colorectal Cancer via mTORC1Activation[J].Cancer Dis, 2014, 4 (12):1366.