PTEN和P53及P16在不同形态大肠侧向发育型肿瘤中的表达

陈健; 张月晓; 张肖丽; 李萍; 齐月雯; 张雷

doi:10.3969/j.issn.1671-038X.2019.05.11

PTEN和P53及P16在不同形态大肠侧向发育型肿瘤中的表达

- 承德医学院附属医院消化内科, 河北承德 067000
基金项目:
承德市科技支撑项目 (No:201801A042)

承德医学院校级人文社科重点课题 (No:201837)

详细信息

作者简介:
陈健, 女, 硕士, 副教授, 研究方向:消化系统疾病诊治及消化内镜下治疗

通讯作者: 张雷, E-mail:912338879@qq.com

中图分类号: R735.3

收稿日期: 2018-12-19

Expression of PTEN, P53 and P16 in different forms of laterally spreading tumors

- Department of Gastroenterology, Affiliated Hospital of Chengde Medical University, Hebei, Chengde 067000, China

More Information

Corresponding author: ZHANG Lei, E-mail: 912338879@qq.com

Received Date: 19 December 2018

摘要

摘要: [目的]观察抑癌基因PTEN、P53、P16蛋白在大肠侧向发育型肿瘤 (LST) 及其三分型中表达变化, 探讨3者在LST发展进程中作用。[方法]选取经内镜下ESD治疗切除的90例大肠组织为LST组, 另选取同时期80例经内镜下ESD治疗切除的大肠隆起型腺瘤 (PA) 大肠组织为PA组, 以及70例结肠镜检查为全结肠未见器质性病变的患者正常大肠组织为对照组。在内镜下将90例LST分为颗粒均一型 (LST-G-H) 27例、结节混合型 (LST-G-M) 25例及非颗粒型 (LST-NG) 38例, 并比较其临床资料;采用免疫组织化学法 (SP法) 检测PTEN、P53、P16蛋白水平。[结果]LST及其三亚型患者病变直径均明显大于PA患者 (P<0.05) 。LST病变多分布于直肠, PA病变多位于远端结肠, 2者比较差异有统计学意义 (P<0.05) 。LST患者中组织学类型所占比例依次为绒毛状腺瘤、管状绒毛状腺瘤、管状腺瘤、锯齿状腺瘤;PA病变中管状腺瘤所占比例最高, 无锯齿状腺瘤;其中, LST-G-H、LST-G-M中以绒毛状腺瘤为主, LST-NG中管状绒毛状腺瘤所占比例最高。LST癌变率显著高于PA (P<0.05) 。PTEN、P16蛋白表达LST患者最低、PA患者次之、对照组最高;P53蛋白表达LST患者最高、PA患者次之、对照组最低。LST-NG患者PTEN蛋白表达显著低于LST-G-H组、LST-G-M组 (P<0.05) , P53、P16蛋白阳性率明显高于LST-G-H组、LST-G-M组 (P<0.05) 。[结论]PTEN、P16在LST患者大肠组织中表达下调, P53表达上调, 可能参与LST特别是LST-NG发生发展。
- 大肠侧向发育型肿瘤 /
- 内镜技术 /
- PTEN /
- P53 /
- P16
Abstract: [Objective]To observe the expression of PTEN, P53 and P16 proteins in laterally spreading tumor (LST) and their three subtypes, and to explore their roles in the development of LST. [Methods]90 cases of colorectal tissues resected by endoscopic submucosal dissection were selected as group LST, another 80 cases of protruded-type colorectal adenoma (PA) resected by ESD at the same time were selected as PA group, and 70 cases of normal colorectal tissues of patients without organic lesions in the whole colon examined by colonoscopy as control group. 90 cases of LST were divided into 3 groups, 27 cases of granular homogeneous type (LST-G-H) , 25 cases of nodular mixed type (LST-G-M) and 38 cases of non-granular type (LST-NG) and their clinical data were compared; the levels of PTEN, P53 and P16 were detected by immunohistochemical method (SP) . [Results]The lesion diameter of LST and three subtypes patients was significantly larger than that of PA patients (P<0.05) . LST lesions are mostly distributed in the rectum, PA lesions mostly located in distal colon and the difference between them is significant (P<0.05) . The proportion of histological types in LST patients was villous adenoma, tubular villous adenoma, tubular adenoma and serrated adenoma in turn; tubular adenomas accounted for the highest proportion of PA lesions, and there was no serrated adenoma; villous adenoma was the predominant lesion in LST-G-H and LST-G-M, tubular villous adenoma accounts for the highest proportion in LST-NG. The canceration rate of LST was significantly higher than that of PA (P<0.05) . The expression of PTEN and P16 protein in LST patients was the lowest, followed by PA patients and the highest in control group; the expression of P53 protein was highest in LST patients, followed by PA patients and the lowest in control group. The expression of PTEN protein in LST-NG patients was significantly lower than that in LST-G-H and LST-G-M groups (P<0.05) , the positive rates of P53 and P16 protein were significantly higher than those of LST-G-H and LST-G-M groups (P<0.05) . [Conclusion]The expression of PTEN and P16 were down-regulated in colorectal tissues of LST patients, the expression of P53 is up-regulated, they may participate in the occurrence and development of LST, especially LST-NG.
- laterally spreading tumors of the large intestine /
- endoscopic technology /
- PTEN /
- P53 /
- P16

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