Expression and mechanism of CDC25A, CerbB-2 and P27 in gastric mucosa of patients with gastric cancer
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摘要: [目的]研究胃癌患者胃黏膜组织中CDC25A、CerbB-2及P27的表达及作用机制。[方法]选取从2016年3月~2018年3月我院收治的胃癌患者100例进行研究，作为胃癌组。另取同期于我院进行胃镜检查的胃炎患者100例为对照组。分别采集2组患者的胃黏膜组织，采用免疫组织化学SP法检测CDC25A、CerbB-2及P27的表达情况，并分析上述各项指标表达情况与胃癌患者临床病理特征的关系。[结果]胃癌组织中的CDC25A、CerbB-2阳性率相比对照组较高，而P27阳性率相比对照组较低（均P<0.05）。TNM分期为Ⅰ~Ⅱ期、分化程度为中高分化、无淋巴结转移的胃黏膜组织CDC25A阳性率相比TNM分期为Ⅲ~Ⅳ期、分化程度为低分化、有淋巴结转移的胃黏膜组织较低（均P<0.05）。有淋巴结转移为黏膜组织CerbB-2阳性率相比无淋巴结转移胃黏膜组织较高（均P<0.05）。TNM分期为Ⅰ~Ⅱ期、分化程度为中高分化、无淋巴结转移的胃黏膜组织P27阳性率相比TNM分期为Ⅲ~Ⅳ期、分化程度为低分化、有淋巴结转移的胃黏膜组织较高（均P<0.05）。[结论]胃癌患者CDC25A、CerbB-2存在明显高表达，而P27存在显著低表达，且CDC25A、P27表达情况与胃癌患者的TNM分期、分化程度以及淋巴结转移存在密切相关，CerbB-2仅与淋巴结转移有关。Abstract: [Objective] To study and analyze the expression and mechanism of CDC25A, CerbB-2 and P27 in the gastric mucosa of patients with gastric cancer. [Methods] A total of 100 patients with gastric cancer admitted to our hospital from March 2016 to March 2018 were selected for the study, which was recorded as the gastric cancer group. Another 100 patients with gastritis who underwent gastroscopy in our hospital at the same time were taken as the control group. The gastric mucosa tissues of the two groups were collected, and the expression of CDC25A, CerbB-2 and P27 were detected by immunohistochemistry SP method, and the relationship between the expression of the above indicators and the clinical and pathological features of the gastric cancer patients was analyzed. [Results] The positive rates of CDC25A and CerbB-2 in gastric cancer tissues were higher than the control group, while the positive rates of P27 were lower than the control group (all P<0.05).TNM stage was Ⅰ~Ⅱ, degree of differentiation of high differentiation, lymph node metastasis of gastric mucosa tissue CDC25A positive rate compared with TNM stage was Ⅲ~Ⅳ, degree of differentiation of low differentiation, lymph node metastasis of gastric mucosa tissue was lower (all P<0.05).The positive rate of CerbB-2 with lymph node metastasis was higher than that without lymph node metastasis (all P<0.05).TNM stage was Ⅰ~Ⅱ, degree of differentiation of high differentiation, lymph node metastasis of gastric mucosa tissue P27 positive rate compared with TNM stage was Ⅲ~Ⅳ, degree of differentiation of low differentiation, lymph node metastasis of gastric mucosa tissues was higher (all P<0.05).[Conclusion] CDC25A and CerbB-2 were significantly over-expressed in gastric cancer patients, while P27 was significantly low expressed, and CDC25A and P27 were closely related to TNM stage, differentiation degree and lymph node metastasis in gastric cancer patients, while CerbB-2 was only related to lymph node metastasis.
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Key words:
- Gastric cancer /
- CDC25A /
- CerbB-2 /
- P27 /
- Clinicopathologic features
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