Intervention effect of ATRA and OMT on Axin,Gsk-3β and Survivin expression in rat liver cancer
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摘要: [目的]观察ATRA联合OMT干预方式在肝癌大鼠病变进程中对Axin、Gsk-3β、Survivin因子的影响及该干预方式在肝癌疾病治疗中的临床价值。[方法]实验中采用DENA作为诱癌剂制备肝癌模型,选取的120只实验SD大鼠将其平均分为诱癌组、干预组及对照组,通过采取病理切片、免疫组化方式观察不同时期3组大鼠的Axin、Gsk-3β、Survivin因子的表达情况。[结果]诱癌大鼠的Axin阳性细胞到后期Axin阳性细胞降低最低点,在诱癌进程中诱癌组大鼠的Axin阳性细胞面积、着色深度等均明显弱于干预组,Axin阳性表达率差异性显著(P＜0.05);诱癌大鼠的Gsk-3β阳性细胞到13~18周降低最低点,诱癌组大鼠在癌变过程中Gsk-3β阳性细胞均出现明显减少,部分细胞质逐步转移至细胞核,在中、后期诱癌组大鼠肝脏组织中的Gsk-3β阳性细胞下降幅度明显大于干预组(P＜0.05),相同时期诱癌组的Gsk-3β阳性细胞面积明显小于干预组;诱癌大鼠肝组织中的Survivin阳性细胞表达在后期达到最高值,在肝癌病变进程中干预组大鼠的Survivin阳性细胞却明显低于诱癌组(P＜0.05)。[结论]通过给予肝癌大鼠实施ATRA联合OMT干预方式,干预组大鼠病变进程中对Axin、Gsk-3β、Survivin因子表达呈一定规律性变化,肝癌病变进程明显滞后,为临床探讨肝癌发病进程及临床治疗提供参考。Abstract: [Objective]To observe the effect of ATRA combined with OMT intervention on Axin,Gsk-3 bata,Survivin and the clinical value of the intervention in the treatment of hepatocellular carcinoma.[Methods]The DENA was used as cancer inducing agent to prepare the model of hepatocellular carcinoma(HCC).One hundred and twenty Sprague Dawley rats were averagely assigned to induced cancer group,intervention group and control group.The expression of Axin,Gsk-3 beta and Survivin in the three groups was observed by pathology and immunohistochemistry in different periods.[Results]Axin positive cells in induced cancer rats decreased the most at late stage,and the expression of GSK-3 beta showed a similar trend.Survivin expression in induced cancer rats increased in later stage to reach the highest value.In the process of HCC lesions,survivin positive cells of intervention group was significantly lower than that in cancer induced group(P＜0.05).[Conclusion]Through implementation of ATRA combined with OMT intervention,there were certain changes in Axin,Gsk-3 beta and Survivin expression,and the disease process of hepatocellular carcinoma(HCC)obviously lags behind,which provides reference for clinical study of liver cancer and its clinical treatment.
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Key words:
- hepatocellular carcinoma /
- ATRA /
- OMT /
- Axin /
- Gsk-3 beta /
- Survivin
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