c-met siRNA转染对食管鳞癌细胞生物学特性的影响

周莹; 李秀娟; 吐尔根·吾那; 张志强

doi:10.3969/j.issn.1671-038X.2016.11.07

c-met siRNA转染对食管鳞癌细胞生物学特性的影响

- 1. 新疆医科大学第一附属医院消化内科, 新疆乌鲁木齐 830011;
- 2. 新疆医科大学, 新疆乌鲁木齐 830011
基金项目:
新疆自治区重大科技专项计划(No:201430123-1)

详细信息

作者简介:
周莹,女,硕士研究生,研究方向:内科学消化

通讯作者: 张志强,E-mail:doczzq@sina.com

中图分类号: R735.1

收稿日期: 2016-01-29

Effects of c-met siRNA on the biological behavior of esophageal squamous cell carcinoma

- 1. Department of Gastroenterology, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China;
- 2. Xinjiang Medical University, Urumqi 830011, China

More Information

Corresponding author: ZHANG Zhi-qiang, E-mail: doczzq@sina.com

Received Date: 29 January 2016

摘要

摘要: [目的]讨论c-Met在食管鳞癌中的表达;构建针对人c-met基因的RNA干扰(RNAi)慢病毒载体,观察其对高侵袭性食管鳞癌细胞c-met基因的沉默效应以及对细胞生物学行为的影响。[方法]培养食管鳞癌CE81T-4细胞株,构建c-met基因的RNAi慢病毒载体,用荧光定量RT-PCR与Western blotting检测其对c-met的干扰效率,用裸鼠移植瘤模型评估RNA干扰后细胞成瘤能力的影响,用裸鼠皮下食管肿瘤生长曲线、肿瘤组织的苏木精-伊红染色及Western blotting验证c-met表达情况。[结果]成功构建c-met基因的RNAi慢病毒载体,qRT-PCR与Western blotting实验显示其对食管鳞癌CE81T-4细胞c-met mRNA及蛋白的表达的抑制均较明显,裸鼠移植瘤实验显示RNA干扰后细胞的成瘤能力降低,裸鼠皮下食管肿瘤生长曲线、肿瘤组织的苏木精-伊红染色、Western blotting实验显示裸鼠移植瘤体内c-met的表达抑制较明显。[结论]c-metRNAi慢病毒载体能够抑制食管鳞癌CE81T-4细胞的侵袭及成瘤能力。
- c-metsiRNA转染 /
- 食管鳞癌 /
- 细胞生物学
Abstract: [Objective]To evaluate the expression of c-Met in esophageal squamous cell carcinoma by constructing lentiviral vectors for RNA interference(RNA)of c-met gene and detecting its silencing effect on the biological behaviors of human esophageal squamous cell carcinoma CE81T-4 cells.[Methods]Human esophageal squamous cell carcinoma CE81T-4 cells were cultured.Lentiviral for RNA of c-met gene was constructed and the silencing effect was detected by RT-PCR and Western blotting.In vivo Tumorigenicity assay was performed to analyze in vivo proliferation of esophageal squamous cell carcinoma CE81T-4 cells in a xenograft model.[Results]Lentiviral RNAi vectors targeting c-met gene were successfully constructed,and they efficiently inhibited the expression of c-met at mRNA and protein levels.Transfection of c-met lentivial RNAi vectors inhibited the invasion and tumorigenicity of CE81T-4 cells.[Conclusion]Lentivirus-mediated c-met RNAi efficiently inhibits invasion and tumorigenicity of CE81T-4 cells.
- c-met /
- siRNA transfection /
- squamocellular cancer of esophagus

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