Clinical value of serum VEGF and VEGFR-2 detection in evaluation the efficacy of neoadjuvant chemotherapy for rectal cancer
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摘要: [目的]探讨血清VEGF与VEGFR-2检测对评估直肠癌新辅助化疗疗效的临床价值。[方法]选择2013年7月~2014年7月医院收治的55例直肠癌患者作为研究组,患者采用奥沙利铂、亚叶酸钙和氟尿嘧啶组成的FOLFOX6方案,患者均完成2个疗程的治疗;另选择同期在医院体检的50例健康人群作为对照组,采用酶联免疫吸附试验检测两组血清VEGF、VEGFR-2水平表达水平。[结果]中分化、高分化组血清VEGF、VEGFR-2水平显著低于低分化组,高分化组血清VEGF、VEGFR-2水平又显著低于中分化组(P＜0.05);Ⅳ期直肠癌患者血清VEGF、VEGFR-2水平显著高于Ⅲ期(P＜0.05)。化疗前研究组血清VEGF、VEGFR-2水平均显著高于对照组,化疗后CR+PR组、SD组血清VEGF、VEGFR-2水平显著降低,PD组血清VEGF、VEGFR-2水平显著升高,化疗前后比较差异具有统计学意义(P＜0.05);其中化疗后CR+PR组、SD组血清VEGF、VEGFR-2显著低于PD组,CR+PR组血清VEGF、VEGFR-2又显著低于SD组(P＜0.05)。相关性分析显示研究组化疗前后血清VEGF、VEGFR-2表达均呈正相关关系(P＜0.05)。[结论]VEGF、VEGFR-2是直肠癌发生、发展的敏感性生物学指标,通过检测患者血清VEGF、VEGFR-2水平能够有助于评价辅助化疗疗效及预后。Abstract: [Objective] To explore the clinical value of serum VEGF and VEGFR-2 detection in evaluating the efficacy of neoadjuvant chemotherapy for rectal cancer.[Methods] A total of 55 cases of rectal cancer patients treated in hospital from July 2013 to July 2014 were selected as study group,constituted by oxaliplatin,leucovorin and fluorouracil of FOLFOX6 therapeutic regimen were treated for patients.All patients received 2 courses of treatment.Another 50 healthy people were selected in same period as the control group,and the levels of serum VEGF and VEGFR-2 expression were detected by enzyme-linked immunosorbent assay in two group.[Results] The levels of serum VEGF and VEGFR-2 in medium differentiation group and highly differentiation group were significantly lower than those in the low differentiation group,and the levels of serum VEGFR-2 and VEGF in highly differentiation group were significantly lower than medium differentiation group(P<0.05).The levels of serum VEGF and VEGFR-2 in Ⅳ stage were obviously higher than those in III stage(P<0.05).Before chemotherapy the levels of serum VEGF and VEGFR-2 in study group were significantly higher than the control group,after treatment the serum levels of VEGF and VEGFR-2 in CR+PR group and SD group were significantly lower,PD group were significantly increased,before and after chemotherapy,the difference was statistically significant(P<0.05);after chemotherapy the serum VEGF and VEGFR-2 in CR+PR group and SD group were significantly lower than that in PD group,and the serum VEGF and VEGFR-2 in CR+PR group were significantly higher than those in SD group(P<0.05).The correlation analysis showed that the expression of serum VEGF and VEGFR-2 in the study group was positively correlated before and after chemotherapy(P<0.05).[Conclusion] The VEGF,VEGFR-2 were sensitive biological indicator for the occurrence and development of colorectal cancer.By detecting the levels of serum VEGF and VEGFR-2 can be helpful to evaluate clinical efficacy and prognosis of adjuvant chemotherapy.
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[1] ATTENBERGER UI, PILZ LR, MORELLI JN, et al.Multi-parametric MRI of rectal cancer-Do quantitative functional MR measurements correlate with radiologic and pathologic tumor stages[J]? Eur J Radiol, 2014, 83:1036-1043.
[2] YASUDA H, TANAKA K, SAIGUSA S, et al.Elevated CD133, but not VEGF or EGFR, as a predictive marker of distant recurrence after preoperative chemoradiotherapy in rectal cancer[J].Oncol Rep, 2009, 22:709-717.
[3] 张蓓, 刘志苏, 黄汉涛, 等.碳酸酐酶Ⅸ在直肠癌中的表达及其与血管内皮生长因子和肝转移关系[J].中华实验外科杂志, 2010, 27(12):1898-1900.
[4] 刘晓平, 邓伟, 苏晋捷, 等.血管内皮生长因子C和Podoplanin对老年直肠癌淋巴结转移预后评价的价值[J].肿瘤防治研究, 2015, 42(2):164-167.
[5] 杨学宁, 吴一龙.实体瘤治疗疗效评价标准-RECIST[J].循证医学, 2004, 4(2):85-90, 111.
[6] DOI H, BEPPU N, ODAWARA S, et al.Neoadjuvant short-course hyperfractionated accelerated radiotherapy(SC-HART)combined with S-1 for locally advanced rectal cancer[J].J Radiat Res, 2013, 54:1118-1124.
[7] 吴文铭, 邱辉忠, 吴斌, 等.临床病理指标预测直肠癌新辅助治疗效果初探[J].中华外科杂志, 2010, 48(5):348-352.
[8] 苏传伟.中性粒细胞明胶酶相关载脂蛋白和癌胚抗原在直肠癌诊断和预后中的意义[J].医学综述, 2014, 20(14):2618-2620.
[9] MAIA J, VAZãO H, PEDROSO DC, et al.VEGF-Functionalized Dextran Has Longer intracellular Bioactivity than VEGF in Endothelial Cells[J].Biomacromolecules, 2012, 13:2906-2916.
[10] 刘涛, 张超, 吕伟, 等.VEGF-C在直肠癌中的表达及其意义[J].重庆医学, 2009, 38(5):508-509, 511.
[11] DIAS FJ, ISSA JP, BARBOSA AP, et al.Effects of low-level laser irradiation in ultrastructural morphology, and immunoexpression of VEGF and VEGFR-2 of rat masseter muscle[J].Micron, 2012, 43:237-244.
[12] 符炜, 李爱娜, 王玉兰, 等.CCX-CKR与VEGF-C在人直肠癌中的表达及临床意义[J].安徽医科大学学报, 2012, 47(2):178-180.
[13] 李靖涛, 高春, 赵洪川, 等.CTHRC1和VEGF-C在直肠癌组织中的表达及相关性[J].世界华人消化杂志, 2009, 17(13):1318-1323.
[14] 王铭河, 师英强, 陆洪芬, 等.Ⅱ、Ⅲ期直肠癌组织中COX-2和VEGF-C的表达及其与肿瘤血管生成及预后的关系[J].中国癌症杂志, 2006, 16(7):557-561, 567.
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