健脾清化中药复方对慢性萎缩性胃炎大鼠TLR4/NF-κB/COX-2信号通路的影响

李思汉; 黄铭涵; 黄健; 林平; 林建龙; 钟国栋; 林煜; 吴菱菱

doi:10.3969/j.issn.1671-038X.2016.07.04

健脾清化中药复方对慢性萎缩性胃炎大鼠TLR4/NF-κB/COX-2信号通路的影响

- 1 福建中医药大学, 福建福州 350108;
- 2 福建中医药大学附属第二人民医院脾胃病科, 福建福州 350003;
- 3 福建医科大学教学医院福建省妇幼保健院, 福建福州 350001
基金项目:
福建省卫生厅青年科研项目(No:2013-1-40)

福建省卫生厅中医科研项目(No:wzpw201305)

福建省自然科学基金(No:2015J01403)

福建省中青年教师教育科研项目(No:JA15247)

福建省科技厅重点项目(No:2015Y0023)

详细信息

作者简介:
李思汉,男,硕士研究生,研究方向:脾胃病的基础与临床研究

通讯作者: 黄铭涵,E-mail:huangminghan2010@163.com

中图分类号: R573.3

收稿日期: 2016-04-09

Effects of Jianpi Qinghua TCM compound on the TLR4/NF-κB/COX-2 signaling pathway in rats with chronic atrophic gastritis

- 1 Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;
- 2 Department of Digestive, the Second People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou 350003, China;
- 3 Maternity and Child Care Centers in Fujian Province, Fuzhou 350001, China

More Information

Corresponding author: HUANG Ming-han, E-mail: huangminghan2010@163.com

Received Date: 09 April 2016

摘要

摘要: [目的]探讨健脾清化中药复方对慢性萎缩性胃炎(chronic atrophic gastritis,CAG)大鼠TLR4/NF-κB/COX-2信号通路的影响。[方法]wistar大鼠随机分为空白组、造模组。造模组大鼠前12周利用20 mmol/L去氧胆酸钠溶液灌胃(2 ml/次/d)联合60%乙醇空腹灌胃(每次2 ml,2次/周)及0.5 g/L氨水自由饮用,建立CAG大鼠模型。确认造模成功后,将造模组大鼠随机分为模型组、中药低剂量组、中药高剂量组各8只;将空白组大鼠随机分为空白对照组和空白中药组各8只。中药低剂量、高剂量组、空白中药组分别按照1.5 ml/kg、6 ml/kg、6 ml/kg的健脾清化复方原液剂量并予生理盐水补齐至4 ml灌胃,空白对照组及CAG模型组给予生理盐水4 ml灌胃,均1次/d,给药30 d。观察各组大鼠的胃组织病理组织学变化,以及对TLR4、NF-κB、COX-2、MYD88 mRNA表达量的影响。[结果]中药干预组胃黏膜萎缩情况明显好转,与模型组比较显著差异(P＜0.01),以中药高剂量组疗效更为显著。与空白组比较,模型组、中药高、低剂量组TLR4、NF-κB、COX-2、MYD88 mRNA水平均明显增高(P＜0.01)。同模型组比较,中药高、低剂量组TLR4、NF-κB、COX-2、MYD88 mRNA水平有下调(P＜0.01),特别以中药高剂量组表现明显。[结论]健脾清化中药复方对CAG大鼠胃黏膜组织病理有明显改善作用,并可有效阻断TLR4/NF-κB/COX-2信号通路的持续活化。
- 健脾清化中药复方 /
- 慢性萎缩性胃炎 /
- TLR4/NF-κB/COX-2信号通路
Abstract: [Objective] To observe the effects of Jianpi Qinghua TCM compound on the activity of the TLR4,NF-κB,COX-2 and MYD88 signaling pathway in rats with chronic atrophic gastritis(CAG).[Methods] CAG rat models were established by intragastric administration with deoxycholic acid sodium salt(2 ml once a day)with concentration of 20 mmol/L,60% ethanol(2 ml twice a week)lavaging on an empty stomach and giving 0.5 g/L aqueous ammonia to drink freely for 12 weeks.CAG models were divided into 3 groups at random:model group(MG),low-dose group of Chinese medicine(LDG)and high-dose group of Chinese medicine(HDG),8 rats per group.Normal rats were randomly divided into blank control group(BCG)and blank control group of Chinese medicine(BCGCM),8 rats per group.1.5 ml/kg,6 ml/kg and 6 ml/kg of Jianpi Qinghua TCM compound were given to the LDG,HDG and BCGCM with the dose and up to 4 ml with normal saline,once a day for 30 days.Respectively,animals in BCG and MG were only given 4 ml normal saline.Pathological changes of the rats stomach tissue were observed and the expression of TLR4,NF-κB,COX-2 and MYD88 mRNA was measured respectively.[Results] The gastric mucosal atrophy in the groups treated with Jianpi Qinghua TCM compound improved markedly,showing significant difference as compared with that in the MG(P＜0.01).The improvement in the HDG was the best.Besides,compared with the BCG,the expression of TLR4,NF-κB,COX-2,MYD88 mRNA increased especially in the MG,LDG and HDG,showing statistical significance(P＜0.01).Compared with the MG,the expression of TLR4,NF-κB,COX-2 and MYD88 mRNA reduced in the LDG and HDG,showing statistical significance(P＜0.01).[Conclusion] Jianpi Qinghua TCM compound had siginificant effect on improving the pathological changes of gastric mucosa.Besides,it could inhibit the continuous activation of TLR4,NF-κB,COX-2 signal pathway.
- Jianpi Qinghua TCM compound /
- CAG /
- TLR4,NF-κB,COX-2 signaling pathway

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