旋覆代赭汤对RE模型大鼠食管组织线粒体超微结构及SDH活性的影响

李姿; 韩慧; 杨幼新; 许云姣; 李美; 袁红霞

doi:10.3969/j.issn.1671-038X.2016.07.03

旋覆代赭汤对RE模型大鼠食管组织线粒体超微结构及SDH活性的影响

- 1 天津中医药大学, 天津 300193;
- 2 天津中医药大学附属北辰中医院中医内科, 天津 300400
基金项目:
国家自然科学基金面上项目(No:81173243)

详细信息

作者简介:
李姿,女,硕士,研究方向:中医内科脾胃病

通讯作者: 杨幼新,E-mail:youxin_yang@hotmail.com

中图分类号: R571

收稿日期: 2015-10-20

Effects of Xuanfu Daizhe Decoction on SDH activity and ultrastructure of esophageal mitochondria in reflux esophagitis rats

- 1 Tianjin Traditional Chinese Medical University, Tianjin 300193, China;
- 2 Beichen Traditional Chinese Medical Hospital Affiliated to Tianjin Traditional Chinese Medical University, Tianjin 300400, China

More Information

Corresponding author: YANG You-xin, E-mail: youxin_yang@hotmail.com

Received Date: 20 October 2015

摘要

摘要: [目的]通过分析旋覆代赭汤及其拆方对反流性食管炎(RE)模型大鼠食管组织线粒体超微结构及琥珀酸脱氢酶(SDH)活性的影响,从微观角度探讨线粒体能量代谢与脾胃气机升降的相关性,阐释RE关键病机及旋覆代赭汤治疗RE的作用机理。[方法]将84只雄性Wistar大鼠随机分为7组,即正常对照组、模型对照组、旋覆代赭汤全方组及拆方各组(包括苦降组、甘升组、升降相因组)、西药组,每组12只。采用"4.2 mm幽门夹+胃底2/3结扎术"制备酸碱混合反流性食管炎动物模型。从术后第8天开始,正常对照组、模型对照组给予生理盐水灌胃,旋覆代赭汤全方组及拆方各组分别给予相应药液,西药组给予(兰索拉唑+莫沙必利)灌胃,干预14 d后,第22天全部处死,利用肉眼及光学显微镜观察食管下段黏膜组织形态学变化,应用电镜观察食管下段黏膜组织线粒体超微结构变化,应用化学法测定食管组织SDH活性的表达情况。[结论]旋覆代赭汤各组分药物都可通过一定途径调节食管组织线粒体能量代谢,甘升组可促进线粒体能量代谢,凡是含有甘升组皆起正性作用,因甘升组药物可促进细胞的新陈代谢,得"扶正益气,健脾化源"之功。旋覆代赭汤通过调节脾胃气机升降,提高食管组织线粒体中SDH的活性,促进线粒体能量代谢,增加线粒体数量,减少线粒体结构及食管黏膜损伤,证实了气机升降与线粒体能量代谢的相关性,从线粒体能量代谢角度揭示了旋覆代赭汤治疗RE的作用机制及途径,并进一步证实RE的关键病机是"胃虚气逆"。
- 反流性食管炎 /
- 旋覆代赭汤 /
- 琥珀酸脱氢酶 /
- 线粒体能量代谢
Abstract: [Objective] To elucidate the key pathogenesis of reflux esophagitis(RE)and the mechanism of Xuanfu Daizhe Decoction(XDD) on it by analyzing the effects of XDD and its separated prescription on esophageal mitochondria ultrastructure and SDH activity in model rats.[Methods] Eighty-four male Wistar rats were randomly divided into 7 groups(n=12 in each),named the normal control group,the model control group,the whole XDD group,and separated prescription of XDD group including the Ku Jiang group,the Gan Sheng group,the Sheng Jiang Xiang Yin group,and the western medicine group treated by Lansoprazole and Mosapride.The mixed acid and alkali RE models were prepared by the 4.2 mm pylorus clamp and two-thirds of gastric fundus ligation.Eight days after operation,the normal control group and model control group were administrated saline,the XDD group and the each separated prescription group were given corresponding medicine.The medicine group were administrated with lansoprazole and mosapride intragastricly,fourteen days after intervention,all of the rats were executed at 22 th day,then the morphological changes of the lower part of esophageal mucosa tissue were observed by naked eyes and optical microscope,meanwhile,the esophageal mitochondria ultrastructural changes of the lower part of esophageal mucosa tissue were observed by electron microscopy.The expression of SDH-activity in esophageal tissue were detected by chemistry method.[Results] The components of the XDD regulated the mitochondrial energy metabolism of esophageal tissue through a certain way.In Gan Sheng group,the generation of ATP was promoted in mitochondrial energy metabolism,and all drugs which contained Gan Sheng group played a positive role.Decoction regulated the qi of spleen and stomach ascending-descending to increase the activity of SDH in the esophageal tissue mitochondria,and promoted the generation metabolism of mitochondrial energy ATP,increased the number of mitochondria,and reduced the damage of mitochondrial structure and esophageal mucosa.[Conclusion] It confirmed the correlation of Qi ascending-descending and mitochondrial energy metabolism,and revealed the mechanism and pathway that the XDD in treating RE mitochondrial energy metabolism,and further affirmed that the key pathogenesis of RE is Qi ascending and stomach deficiency.
- Reflux esophagitis /
- Xuanfu Daizhe Decoction /
- succinate dehydrogenase /
- mitochondrial energy metabolism

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