消溃平液抗胃癌癌前病变的实验研究

赖四海; 吴建维; 陈小燕; 郭玉庆; 郑宵月

doi:10.3969/j.issn.1671-038X.2015.07.06

消溃平液抗胃癌癌前病变的实验研究

- 深圳市第八人民医院消化内科, 深圳 518101

详细信息

通讯作者: 赖四海,E-mail:godai@126.com

中图分类号: R735.2

收稿日期: 2015-02-01

Experimental study of Xiaokuiping liquid of anti-precancerous lesions in gastric cancer

- Department of Gastroenterology, the Eighth People's Hospital of Shenzhen City, Shenzhen 518101, China

More Information

Corresponding author: LAI Si-hai, E-mail: godai@126.com

Received Date: 01 February 2015

摘要

摘要: [目的]通过动物实验观察消溃平液抗胃黏膜细胞突变、癌变的作用。[方法]①应用化学致癌快速筛选短期实验的细胞非程序合成(UDS)的方法,做大鼠体内和体外突变实验,观察消溃平液对化学致癌物MNNG诱导的大鼠外周血白细胞突变的阻断突变作用。②用小鼠90只,分为3个组,1组饮自来水,2组自由饮含150 ug/ml致癌物—MNNG的自来水,3组自由饮含150 ug/ml MNNG加含25 mg/ml消溃平液自来水,2周后处死动物,胃切片病理诊断癌变。结果①体内诱导实验,大鼠经2周饲养诱导UDS值从4120.31±188.22降到3151.28±82.91,P＜0.01;体外诱导血中白细胞UDS值由4187.22±592.42下降到3102.23±306.58,P＜0.01;②小鼠饮含致癌物MNNG组30只中17只发生了胃黏膜癌变,而饮用MNNG同时加饮25 ml/l消溃平液30只中5只发生癌变,抑癌率70.58%。[结论]消溃平液能抑制致癌物MNNG对大鼠外周血白细胞诱发突变的作用;对小鼠胃黏膜增生癌变有高效的抑制作用。
- 消溃平液 /
- DNA非程序合成 /
- MNNG /
- 白细胞 /
- 胃黏膜增生癌变
Abstract: [Objective] To observe the role of Xiaokuiping liquid in anti-mutation and anti-canceration by animal experiment.[Methods] (1)The effect of Xiaokuiping liquid on rat peripheral blood leukocytes,which was induced mutation by chemical carcinogens MNNG,was observed in rat mutation experiments in vivo and in vitro based on the method of unscheduled DNA synthesis(UDS)test of chemical carcinogenesis rapid screening short-term experiment.(2)90 mice were divided into 3 groups.No.1 group mice were given tap water.No.2 group mice were given 150 ug/ml carcinogens-MNNG diluted with tap water.No.3 group mice were given MNNG and 25 mg/ml Xiaokuiping liquid diluted with tap water.After 2 weeks,the animals were sacrificed and the stomach histopathology was examined to diagnose canceration.[Results] (1)In vivo induction experiments,the UDS value was decreased from 4120.31±188.22 to 3151.28±82.91(P<0.01).In vitro,the UDS value of blood leukocytes was decreased from 4187.22±592.42 to 3102.23±306.58(P<0.01).(2)17 mice of No.2 group were found canceration in gastric mucosae in contrast to the 5 mice of No.3 group.The tumor-inhibition rate was 70.58%.[Conclusion] Xiaokuiping liquid can inhibit the mutation of rat peripheral blood leukocytes induced by chemical carcinogens MNNG,and can effectively inhibit the hyperplasia and canceration of the mouse gastric mucosa.
- Xiaokuiping liquid /
- unscheduled DNA synthesis /
- MNNG /
- leukocyte /
- gastric mucosa hyperplasia and canceration

HTML全文

参考文献(14)

[1]	MING S C,BAJTAI A,CORREA P,et al.Gastric dysplasia.Significance and pathologic criteria[J].Cancer,1984,54:1794-1801.
[2]	LI C Q,LIU W W.Intestinal metaplasia,dysplasia and gastric cancer:a study of mucohistochemistry,immunohistochemistry and cell DNA quantitative analysis[J].J Medical Coll PLA,1992,7:192-196.
[3]	SARAGA E P,GARDIOL D,COSTA J.Gastric dysplasia.A histological follow-up study[J].Am J Surg Pathol,1987,11:788-796.
[4]	李琼,刘晏,吴坚炯.慢性萎缩性胃炎的治疗进展[J].中医学报,2013,28(176):114-115.
[5]	诸亚君,陈望秋,曹禄森,等.富硒麦芽粉对黄曲霉毒素诱导外周血白细胞DNA非程序合成的影响[J].营养学报,1990,12(3):248-252.
[6]	王刚,赵钦.两种白茶的抗突变和体外抗癌效果[J].食品科学,2009,30(11):240-242.
[7]	周杰,朱萱萱,王海丹,等.黄芪多糖对人胃癌细胞系MGC-803的生长抑制作用及细胞周期的影响[J].中华中医药学刊,2013,31(5):1116-1119.
[8]	张秋菊,刘斌.基于细胞凋亡机制的黄芪抗肿瘤作用研究进展[J].中国老年医学杂志,2013,33(11):2729-2731.
[9]	许波,叶柏.中医药治疗胃癌癌前病变的研究进展[J].光明中医,2013,28(4):858-862.
[10]	陈光,臧文臣,刘显清,等.黄芪多糖对动物肿瘤细胞凋亡影响的研究[J].中医药学报,2002,30(4):55-56.
[11]	刘鹏熙,周瑞芳,谭敏.黄芪注射液对basa-l like型乳腺癌MDA-MB-231细胞株增殖和凋亡的影响[J].中华乳腺病杂志(电子版),2009,3(2):181-186.
[12]	孙聪,韩业超,洪敏,等.黄芪多糖抑制大鼠神经胶质瘤细胞增殖的实验研究[J].中国老年学杂志,2009,29(1):41-43
[13]	申海涛,张祥宏,郑末,等.经口给予黄芪提取物对H22肝癌荷瘤小鼠影响的实验研究[J].中国肿瘤临床,2006,33(14):821-824.
[14]	曹洁,李瑞珍,史永亮,等.亚硒酸钠和维甲酸对MNNG诱导人全血白细胞非程序DNA合成的影响[J].癌变畸变突变,1998,10(1):15-17.