Foxo3a激活FasL参与胃黏膜上皮细胞凋亡探讨舒胃汤防治肝胃郁热型胃溃疡的研究

张琪; 梁海业; 陈世新; 李丽清

doi:10.3969/j.issn.1671-038X.2015.07.03

Foxo3a激活FasL参与胃黏膜上皮细胞凋亡探讨舒胃汤防治肝胃郁热型胃溃疡的研究

- 广西桂平市中医医院脾胃病科, 广西桂平 537200
基金项目:
广西卫生厅自筹经费项目(No:Z2014554)

详细信息

作者简介:
张琪,女,本科,副主任中医师,主要从事消化性溃疡中医药防治。

通讯作者: 张琪,女,E-mail:327664246@qq.com

中图分类号: R573.1

收稿日期: 2015-01-15

Clinical study on Shuwei decoction in the prevention and treatment of gastric ulcer with heat stagnancy of liver and stomach through Foxo3a activated FasL mediating gastric epithelial cell apoptosis

- Department of Spleen Stomach Disease, The TCM Hospital of Guiping City, Guiping 537200, China

More Information

Corresponding author: ZHANG Qi, E-mail: 327664246@qq.com

Received Date: 15 January 2015

摘要

摘要: [目的]通过检测治疗前、后胃黏膜上皮细胞凋亡、Foxo3a、FasL及胃镜变化,探讨舒胃汤防治胃溃疡的可能机制。[方法]选择符合诊断标准、纳入标准及排除标准的胃溃疡患者30例,予舒胃汤连续6周。应用电子胃镜检测治疗前、后溃疡变化;TUNEL染色检测治疗前后胃黏膜上皮细胞凋亡变化;以及Real-time PCR技术检测治疗前后Foxo3a、FasL、Caspase-8变化。[结果]从胃镜下溃疡修复分析,舒胃汤治疗肝胃郁热型胃溃疡总有效率为80%,治疗前胃黏膜上皮细胞凋亡率为14.36±2.71%,Foxo3a、FasL及Caspase-8 mRNA表达分别为1.89±0.32、2.13±0.25、2.04±0.22;治疗后胃黏膜上皮细胞凋亡率为5.73±0.46%,Foxo3a、FasL及Caspase-8 mRNA表达分别为1.02±0.11、1.13±0.19、1.21±0.09。[结论]舒胃汤可能通过下调Foxo3a、FasL及Caspase-8表达,延缓上皮细胞凋亡,促进黏膜修复,达到防治胃溃疡的效用。
- 舒胃汤 /
- Foxo3a/FasL /
- 胃黏膜上皮细胞凋亡 /
- 胃溃疡
Abstract: [Objective] To access the prevention mechanism of Shuwei decoction on gastric ulcer by detecting the apoptosis of gastric mucosa epithelial cells and the expression of Foxo3a,FasL and the changes under gastroscope before and after the treatment.[Methods] Thirty gastric ulcer patients who matched the diagnostic criteria,inclusion criteria and exclusion criteria were given Shuwei decoction for 6 weeks.The changes of ulcer were observed under the gastroscope before and after the treatment.The apoptosis of gastric mucosal epithelial cells was detected by TUNEL staining.The expression of Foxo3a,FasL and Caspase-8 were detected by Real-time PCR.[Results] Based on the analysis of ulcer repair under gastroscope,the total efficiency of Shuwei decoction was 80%.Before the treatment,the apoptosis rate of gastric mucosa epithelial cells was 14.36±2.71%.The expression of Foxo3a,FasL and Caspase-8 mRNA were 1.89±0.32.2.13±0.25.2.04±0.22,respectively.But after the treatment,the apoptosis rate of gastric mucosa epithelial cells was 5.73±0.46%.The expression of Foxo3a,FasL and Caspase-8 mRNA were 1.02±0.11,1.13±0.19,1.21±0.09 respectively.[Conclusion] Shuwei decoction can prevent the occurrence of gastric ulcer by reducing the expression of Foxo3a,FasL and Caspase-8 , delaying the epithelial cell apoptosis and promote mucosal repair.
- Shuwei decoction /
- Foxo3a/FasL /
- gastric epithelial cell apoptosis /
- gastric ulcer

HTML全文

参考文献(14)

[1]	SUGIMOTO M,UOTANI T,FURUTA T.Does rebamipide prevent gastric mucosal injury in patients taking aspirin and clopidogrel[J]?Dig Dis Sci.,2014,59:1671-1673.
[2]	GONG M,LING S S,LUI S Y,et al.Helicobacter pylori gamma-glutamyl transpeptidase is a pathogenic factor in the development of peptic ulcer disease[J].Gastroenterology,2010,139:564-573.
[3]	LUO X J,LIU B,DAI Z,et al.Stimulation of calcitonin gene-related peptide release through targeting capsaicin receptor:a potential strategy for gastric mucosal protection[J].Dig Dis Sci,2013,58:320-325.
[4]	KOBAYASHI M,TAKEUCHI M,HASHIMOTO S,et al.Contributing factors to gastric ulcer healing after endoscopic submucosal dissection including the promoting effect of rebamipide[J].Dig Dis Sci,2012,57:119-126.
[5]	HUANG P,ZHOU Z,WANG H,et al.Effect of the IGF-1/PTEN/Akt/FoxO signaling pathway on the development and healing of water immersion and restraint stress-induced gastric ulcers in rats[J].Int J Mol Med,2012,30:650-658.
[6]	XIA H H,TALLEY N J.Apoptosis in gastric epithelium induced by Helicobacter pylori infection:implications in gastric carcinogenesis[J].Am J Gastroenterol,2001,96:16-26.
[7]	葛均波,徐永健主编.内科学[M].8版.北京:人民卫生出版社,2013:347-350.
[8]	中华中医药学会.中医内科学常见病诊治指南,西医疾病部分[M].北京:中国中医药出版社,2008:97-99.
[9]	中国中西医结合学会消化系统疾病专业委员会.消化性溃疡中西医结合诊疗共识意见[J].中国中西医结合消化杂志,2012,32(6):733-739.
[10]	LV G,ZHU H,ZHOU F,et al.AMP-activated protein kinase activation protects gastric epithelial cells from Helicobacter pylori-induced apoptosis[J].Biochem Biophys Res Commun,2014,453:13-18.
[11]	ZHU W L,TONG H,TEH J T.Forkhead box protein o3 transcription factor negatively regulates autophagy in human cancer cells by inhibiting forkhead box protein o1 expression and cytosolic accumulation[J].PLoS One,2014,9:e115087.
[12]	McCRACKEN K W,CATÁE M,CRAWFORD C M,et al.Modelling human development and disease in pluripotent stem-cell-derived gastric organoids[J].Nature,2014,516:400-404.
[13]	ZAIDI S F,MUHAMMAD J S,SHAHRYAR S,et al.Anti-inflammatory and cytoprotective effects of selected Pakistani medicinal plants in Helicobacter pylori-infected gastric epithelial cells[J].J Ethnopharmacol,2012,141:403-410.
[14]	WANG J,LIU S,YIN Y,et al.FOXO3-mediated up-regulation of Bim contributes to rhein-induced cancer cell apoptosis[J].Apoptosis,2014,[Epub ahead of print]