Rat model of functional dyspepsia resulting from iodoacetamide-treated and tail-squeezed
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摘要: [目的]采用碘乙酰胺(iodoacetamide,IA)灌胃刺激联合夹尾应激法建立功能性消化不良(functional dyspepsia,FD)大鼠模型,并对其进行初步评价。[方法]18只10日龄SD大鼠随机分为正常组(9只)和模型组(9只)。正常组大鼠每只给予2%蔗糖溶液0.2 ml/d灌胃,持续6 d;模型组大鼠每只给予0.1%的碘乙酰胺与2%蔗糖的混合液0.2 ml/d灌胃,持续6 d。灌胃结束后各组大鼠正常饲养至7周龄,7周龄起以郭氏夹尾刺激法对模型组大鼠刺激1周,正常组不予任何干预,直至大鼠8周龄。大鼠8周龄时检测大鼠胃敏感性(以胃扩张刺激时大鼠颈斜方肌肌电均方根变化率为评价指标)及顺应性(以胃扩张刺激时大鼠胃内球囊容积/压力为评价指标),并于术后第二天检测各组大鼠3 h摄食量及胃排空率。[结果]在IA灌胃期间(大鼠10日龄至15日龄),模型组大鼠体重增长率较正常组显著降低(P<0.05)。大鼠7周龄时,各组大鼠体重无显著差异(P>0.05)。7周龄开始予夹尾刺激1周后(大鼠8周龄),模型组大鼠体重较正常组显著降低,结果具有统计学意义(P<0.05)。大鼠8周龄时胃敏感性及顺应性检测结果显示:在胃内球囊压力为40-60 mmHg时,模型组大鼠与正常组大鼠相比较,颈斜方肌肌电均方根变化率显著升高(P<0.05),胃顺应性显著降低(P<0.05)。胃排空率结果显示:模型组大鼠胃排空率较正常组大鼠显著降低(P<0.05)。[结论]采用IA灌胃法与夹尾应激法相结合的方法建立FD大鼠模型可在一定程度上模拟FD的临床症状及内脏高敏感、胃顺应性降低、胃排空延缓等病理状态。Abstract: [Objective] To establish a rat model of functional dyspepsia (FD) resulting from iodoacetamide(IA)-treated and tail-squeezed.[Methods] 18 ten-day-old male rats were randomly divided into two groups. The model rats(n=9) received 0.2 mL 0.1%iodoacetamide (IA) in 2% sucrose daily by oral gavages for 6 days; controls(n=9) received 2% sucrose. Rats in both groups were then followed to 7weeks at which point the model rats received tail-squeezed for 1 week. Then gastric accommodation and acromiotrapezius(a superficial neck muscle) responses to graded gastric balloon distention (GD; 10-60 mm Hg) were tested, and gastric motor function was tested after that.[Results] Model rats exhibited symptoms of FD as compared with the control group. The gastric accommodation was significantly reduced and acromiotrapezius responses to GD were significantly increased in IA-treated rats. Gastric emptying of model rats was significantly lower than controls. However, no significant gastric pathology was found in both groups.[Conclusion] Our results provide a potential model for further study to functional dyspepsia which simulates the pathogenesis and symptoms of this important clinical problem.
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[1] TACK J,TALLEY N J,CAMILLERI M,et al.Functional Gastroduodenal Disorders[J].Gastroenterology,2006,130:1466-1479.
[2] El-Serag H B,Talley N JEL-SERAG H B,TALLEY N J.Systemic review:the prevalence and clinical course of functional dyspepsia[J].Aliment Pharmacol Ther,2004,19:643-654.
[3] LI Y,NIE Y,SHA W,et al.The link between psychosocial factors and functional dyspepsia:an epidemiological study[J].Chin Med J(Engl),2002,115:1082-1084.
[4] LIU L S,WINSTON J H,SHENOY M M,et al.A rat model of chronic gastric sensorimotor dysfunction resulting from transient neonatal gastric irritation[J].Gastroenterology,2008,134:2070-2079.
[5] RÖMER M,PAINSIPP E,SCHWETZ I,et al.Facilitation of gastric compliance and cardiovascular reaction by repeated isobaric distension of the rat stomach[J].Neurogastroenterol Motil,2005,17:399-409.
[6] Wei W,Li X,Hao J,et al.Proteomic analysis of functional dyspepsia in stressed rats treated with traditional Chinese medicine"Wei Kangning"[J].J Gastroenterol Hepatol,2011,26:1425-1433.
[7] 郭海军,林洁,李国成,等.功能性消化不良的动物模型研究[J].中国中西医结合消化杂志,2001,9(3):141-142.
[8] TU W Z,CHENG R D,CHENG B,et al.Analgesic effect of electro acupuncture on chronic neuropathic pain mediated by P2 X3 receptors in rat dorsal root ganglion neurons[J].Neurochemistry International,2012,60:379-386.
[9] MARTINEZ V,BARQUIST E,RIVIER J,et al.Central CRF inhibits gastric emptying of a nutrient solid meal in rats:the role of CRF2 receptors[J].Am J Physiol,1998,274:G965-970.
[10] 肖政华.四磨汤对功能性消化不良患者的临床疗效及模型小鼠Ghrelin、GHSR表达的影响[D].湖南长沙:湖南中医药大学,2012:46.-46.
[11] 刘柏炎,易健,蔺晓源,等.四磨汤对慢性应激小鼠多巴胺受体D1,D2的影响[J].中国实验方剂学杂志,2011,17(3):193-193.
[12] CAMILERRY M,COULIE B,TACK J F.Visceral hypersensitivity:facts,speculations,and challenges[J].Gut,2001,48:125-131.
[13] BOECKXSTAENS G E,HIRSCH D P,KUIKEN S D,et al.The proximal stomachand postprandial symptoms in functional dyspeptics[J].Am J Gstroenterol,2002,97:40-48.
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