参灵合剂对原发性肝癌小鼠抗肿瘤免疫反应的实验研究

孙丽红; 王海颖

doi:10.3969/j.issn.1671-038X.2015.05.03

参灵合剂对原发性肝癌小鼠抗肿瘤免疫反应的实验研究

孙丽红¹,
王海颖^2, ,

- 1 上海中医药大学公共健康学院, 上海 201203;
- 2 上海中医药大学中药学院, 上海 201203
基金项目:
国家社会科学基金重点项目(No:12AZD094)

上海市教育委员会科研项目(No:09JW15)

国家自然科学基金项目(No:81403345)

上海市教育委员会科研创新项目(No:14YZ059)

详细信息

作者简介:
孙丽红,女,医学博士,副教授,主要从事肿瘤临床治疗和实验研究

通讯作者: 王海颖,E-mail:wanghaiying_7@hotmail.com

中图分类号: R734.7

收稿日期: 2014-12-02

The experimental research of antitumor immune responses of Shenling Mixture on mice with primary liver cancer

SUN Li-hong¹,
WANG Hai-ying^2, ,

- 1 School of Public Health, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;
- 2 Institute of Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

More Information

Corresponding author: WANG Hai-ying, E-mail: wanghaiying_7@hotmail.com

Received Date: 02 December 2014

摘要

摘要: [目的]探讨人参、灵芝、半枝莲和参灵合剂(三药合一)对原发性肝癌小鼠抗肿瘤及其作用机制。[方法]采用H22肝癌荷瘤小鼠模型,按体质量随机分为正常组、对照组、人参组、灵芝组、半枝莲组和参灵合剂组,每组20只。正常组和对照组每日均给予生理盐水20 ml/kg,人参组每日灌胃人参皂苷25 mg/kg,灵芝组每日灌胃灵芝多糖提取物830 mg/kg,半枝莲组每日灌胃半枝莲多糖200 mg/kg,参灵合剂组每日灌胃参灵合剂10 g,各组均持续灌胃10 d。检测药物对胸腺指数、肿瘤坏死因子-α(TNF-α)、白细胞计数和血管内皮生长因子(VEGF)等指标的影响。[结果]与对照组相比,参灵合剂组明显改变肝癌小鼠的脾脏指数、胸腺指数和外周血白细胞计数(P＜0.05,P＜0.01);给药组对TNF-α和VEGF有显著影响(P＜0.05)。[结论]人参皂甙、半枝莲多糖、灵芝多糖提取物和参灵合剂对原发性肝癌小鼠模型都有一定的抑瘤作用,其中参灵合剂抑瘤作用最强。
- 参灵合剂 /
- 原发性肝癌 /
- 抑瘤 /
- 免疫调节
Abstract: [Objective] To discuss the mechanism of action of antitumor effects of ginseng, ganoderma, barbed skullcap herb and Shenling Mixture (containing the above three drugs) on primary liver cancer in mice.[Methods] Mouse models of primary liver cancer were given saline, panaxoside, ganoderma lucidum polysaccharides extract, barbed skullcap herb polysaccharides and the mixture of three drugs,and the index of thymus, TNF-α, white blood cells and VEGF were detected. Mice were randomly divided into normal group(20 ml/kg NS,10 d),control group(20 ml/kg NS,10 d),ginseng group(25 mg/kg panaxoside,10 d),ganoderma group(830 mg/kg Ganoderma lucidum polysaccharides extract,10 d),barbed skullcap herb group(200 mg/kg barbed skullcap herb polysaccharides 10 d),and Shenling Mixture group(10 g of the panaxoside,ganoderma lucidum polysaccharides extract and barbed skullcap herb polysaccharides,10 d),twenty mice in each group.[Results] Shenling Mixture significantly changed the index of thymus and spleen and also the number of white blood cells in peripheral blood compared with saline(P＜0.05, P＜0.01). All the drugs had a significant influence on TNF-α and VEGF(P＜0.05).[Conclusion] Ginsenosides, polysaccharide, ganoderma lucidum polysaccharide and Shenling Mixture had tumor-suppressing effect on tumor-burdened primary liver cancer in mice,and the inhibitory action of Shenling Mixture was the strongest.
- Shenling Mixture /
- primary liver cancer /
- tumor-suppressing /
- immunoregulation

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