雷公藤多苷对溃疡性结肠炎小鼠结肠黏膜TLR4信号通路表达的影响

钟继红; 王章流; 刘英超; 沈雁; 方国栋

doi:10.3969/j.issn.1671-038X.2015.05.02

雷公藤多苷对溃疡性结肠炎小鼠结肠黏膜TLR4信号通路表达的影响

- 浙江中医药大学附属第二医院消化内科, 杭州 310005
基金项目:
浙江省教育厅科研资助项目(No:Y201329099)

详细信息

作者简介:
钟继红,女,硕士,主治中医师,研究方向:中西医结合对消化系疾病的研究

通讯作者: 钟继红,E-mail:49747048@qq.com

中图分类号: R574.62

收稿日期: 2014-12-17

Effect of tripterygium glycosides on the expression of TLR4 in colonic mucosa of ulcerative colitis in mice

- Department of Gastroenterology, the Second Affiliated Hospital of Zhejiang Chinese Medicine University, Hangzhou 310005, China

More Information

Corresponding author: ZHONG Ji-hong, E-mail: 49747048@qq.com

Received Date: 17 December 2014

摘要

摘要: [目的]观察雷公藤多苷对右旋葡聚糖硫酸钠(DSS)诱导的小鼠溃疡性结肠炎模型结肠黏膜LPS/TLR4信号通路表达的影响,探讨其治疗溃疡性结肠炎的可能作用机制。[方法]BALB/c小鼠随机分为6组:模型组,低、中及高剂量中药雷公藤多苷灌胃组、阴性对照组、正常对照组。采用DSS复制BALB/c小鼠溃疡性结肠炎动物模型,雷公藤多苷灌胃给药21 d后,采用荧光定量PCR、western blot法检测TLR4 mRNA和蛋白的表达。[结果]3组中药灌胃组较阳性对照组TLR4表达水平显著降低(P＜0.01),但中、高剂量中药灌胃组、阴性对照组与正常组两两之间表达水平差异无统计学意义(P＞0.05)。[结论]雷公藤多苷能够抑制LPS/TLR4信号通路的表达,抑制UC小鼠的炎症反应,对UC发病起到一定得保护作用。
- 雷公藤多苷 /
- 葡聚糖硫酸钠 /
- 溃疡性结肠炎 /
- TLR4
Abstract: [Objective] To observe the effect of Tripterygium wilfordii on LPS/TLR4 signaling pathway of colon mucosa in dextran sulphate sodium(DSS)induced ulcerative colitis mice, and to explore its possible mechanisms of action. [Methods] BALB/c mice were randomly divided into six groups:model group,low, medium and high dose groups of Chinese medicine tripterygium glycosides intragastric group,negative control group and normal control group. Ulcerative colitis model was made by DSS in BALB/c mice, and treatment was 21 days. TLR4 mRNA was detected by real-time PCR and western blot.[Results] The expression of TLR4 was significantly decreased in the three groups of herbs compared with the positive control group(P＜0.01),but there was no significant difference between the medium and high doses of groups or between negative control group and the normal group (P＞0.05).[Conclusion] Tripterygium glycosides can suppress the LPS/TLR4 signaling pathway,and inhibit the inflammatory reaction in UC mice,thus can be effective in controlling ulcerative colitis.
- tripterygium glycosides /
- dextran sulfate sodium /
- ulcerative colitis /
- TLR4

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