肠道感染中致泻性大肠埃希菌的检测及流行分析

陆清平; 林志强; 梁志军

doi:10.3969/j.issn.1671-038X.2015.04.15

肠道感染中致泻性大肠埃希菌的检测及流行分析

- 琼海市人民医院感染科, 海南琼海 571400

详细信息

作者简介:
陆清平,主治医师,本科,专业:感染病

中图分类号: R373.2

收稿日期: 2014-11-28

Detection and intestinal infections caused by Escherichia coli diarrhea epidemic analysis

- Department of Infections, People's Hospital of Qionghai, Qionghai 571400, China

Received Date: 28 November 2014

摘要

摘要: [目的]检测肠道感染中致泻性大肠埃希菌（DEC）的检出情况，并对疾病流行情况进行分析，为预防和诊治疾病提供依据。[方法]选取2009年5月~2014年5月医院消化门诊腹泻患者841例，所有患者均通过粪便标本以及PCR基因检测方法检测病原菌种类，统计致泻性大肠埃希菌，并结合患者的临床资料，分析其流行情况。[结果]841例标本中检出DEC151例，阳性率为17.95%。其中肠致病性大肠埃细菌（EPEC）22株，肠出血性大肠埃希菌（EHEC）1株，肠产毒性大肠埃希菌（ETEC）64株，肠聚集性大肠埃希菌（EAEC）15株，弥散聚集性大肠埃希菌（DAEC）29株，阳性率分别占14.57%、0.66%、42.38%、9.39%和19.21%。仅eastA基因阳性未能分类20株占13.25%；发病年龄0~13岁患者DEC检出率为9.71%，13~60岁DEC检出率为21.27%，60岁以上DEC检出率为13.37%；DEC好发于夏秋季（6~11月），共检出DEC106例占70.20%。[结论]DEC是导致肠道感染腹泻的主要病原菌之一，DEC在不同季节和不同年龄的人群检出率有所差异，好发于夏秋季，且年龄在13~60岁检出率较高。
- 肠道感染 /
- 致泻性大肠埃希菌 /
- 流行情况
Abstract: [Objective] To detect intestinal infections caused by Escherichia coli diarrhea(DEC) in the detection of cases, and to analyze the prevalence of the disease and provide evidence for the prevention and treatment of disease.[Methods] Selected in May 2009 to May 2014 digest outpatient hospital 841 cases of diarrhea patients, and all patients through genetic testing stool samples and PCR method to detect pathogens, Escherichia coli diarrhea statistics, combined with the patient's clinical data analyze its prevalence.[Results] Eight hundred and forty-one cases were detected in samples DEC151 cases;the positive rate was 17.95%.Which intestinal pathogenic bacteria Escherichia Egypt(EPEC) 22 strains of enterohaemorrhagic Escherichia coli(EHEC) 1 strains of enterotoxaemia Escherichia coli(ETEC) 64 strains of Escherichia coli intestinal aggregation(EAEC) 15, diffuse clusters of Escherichia coli(DAEC) 29 strains positive rate accounted for 14.57%, 0.66%, 42.38%, 9.39% and 19.21% respectively.Only eastA gene positive failed to classify 20 accounted for 13.25%;onset age of 0 to-13 years old DEC detection rate of 9.71% of patients, 13~60 years old DEC detection rate of 21.27%, the detection rate of over 60 years DEC 13.37%;DEC occurs in the summer and autumn(June to November), and were detected DEC106 cases accounted for 70.20%.[Conclusion] DEC is one of the main pathogens cause intestinal infections diarrhea.DEC vary in different seasons and different age groups detection rate, occur in summer and autumn, and have a higher detection rate in the 13-60 year old age.
- intestinal infections /
- diarrhea caused by E.coli /
- prevalence

HTML全文

参考文献(10)

[1]	WILEMAN B W, THOMSON D U, OLSON K C, et al.Escherichia coli O157:H7 shedding in vaccinated beef calves born to cows vaccinated prepartum with Escherichia coli O157:H7 SRP Vaccine[J].Journal of Food Protection, 2011, 74:1599-1604.
[2]	赵雪涛, 高昆, 张春华, 等.2011至2012年徐汇区致泻性大肠埃希菌病原学监测与流行病学研究[J].检验医学, 2013, 28(8):671-675.
[3]	黄峥, 许浩, 郭家胤, 等.4种致泻性大肠埃希菌分子诊断方法的评估及其在监测中的应用[J].中华流行病学杂志, 2013, 34(6):614-617.
[4]	赵爱兰, 熊衍文, 白雪梅, 等.鉴定五类致泻性大肠埃希菌和志贺菌的多重PCR方法[J].疾病监测, 2011, 26(1):65-67.
[5]	SIDHU J P S, AHMED W, HODGERS L, et al.Occurrence of virulence genes associated with diarrheagenic pathotypes in Escherichia coli isolates from surface water[J].Applied and Environmental Microbiology, 2013, 79:328-335.
[6]	TANIUCHI M, WALTERS C C, GRATZ J, et al.Development of a multiplex polymerase chain reaction assay for diarrheagenic Escherichia coli and Shigella spp.and its evaluation on colonies, culture broths, and stool[J].Diagnostic microbiology and infectious disease, 2012, 73:121-128.
[7]	FUJIOKA M, OTOMO Y, AHSAN C R, et al.A novel single-step multiplex polymerase chain reaction assay for the detection of diarrheagenic Escherichia coli[J].Journal of Microbiological Methods, 2013, 92:289-292.
[8]	BYRD W, BOEDEKER E C.Attenuated Escherichia coli strains expressing the colonization factor antigen I(CFA/I)and a detoxified heat-labile enterotoxin(LThK63)enhance clearance of ETEC from the lungs of mice and protect mice from intestinal ETEC colonization and LT-induced fluid accumulation[J].Vet Immunol Immunopathol, 2013, 152:57-67.
[9]	RUND S A, ROHDE H, SONNENBORN U, et al.Antagonistic effects of probiotic Escherichia coli Nissle 1917 on EHEC strains of serotype O104:H4 and O157:H7[J].International Journal of Medical Microbiology, 2013, 303:1-8.
[10]	CHA SEUNGBIN, YOO ANNA, LEE WONJUNG, et al.Effect of bacteriophage in enterotoxigenic Escherichia coli(ETEC)infected pigs[J].The Journal of Veterinary Medical Science, 2012, 74:1037-1039.