灭幽汤对幽门螺杆菌相关性胃炎脾胃湿热证小鼠NF-&#954;B65、HSP70的影响

郭璇; 王小娟; 喻斌; 郭建生; 罗燕; 胡淑娟; 杜中华; 夏蓉; 尹姣

doi:10.3969/j.issn.1671-038X.2014.10.02

灭幽汤对幽门螺杆菌相关性胃炎脾胃湿热证小鼠NF-κB65、HSP70的影响

- 1. 湖南中医药大学, 湖南长沙 410208;
- 2. 湖南中医药大学第一附属医院消化内科, 湖南长沙 410007
基金项目:
湖南省自然科学基金项目（No：12JJ2046）；湖南省科技厅资助科研项目（No：2011SK3096）；湖南省科普计划项目（No：2013KP0111）；湖南省中医药科研计划项目（No：2014165）

详细信息

作者简介:
郭璇,女,硕士研究生,讲师,从事中医内科方面研究

通讯作者: 郭建生,E-mail:360319179@qq.com

中图分类号: R285.5

收稿日期: 2014-06-27

Effect of Mieyou Soup on NF-κB65 and HSP70 in the H.Pylori-associated gastritis mice of Pi-Wei hygropyrexia syndrome（PWHS）

- 1. Hunan University of Chinese Medicine, Changsha 410208, China;
- 2. the First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, China

More Information

Corresponding author: GUO Jian-sheng, E-mail: 360319179@qq.com

Received Date: 27 June 2014

摘要

摘要: [目的]研究灭幽汤对幽门螺杆菌（helicobacter pylori，Hp）相关性胃炎脾胃湿热证小鼠核因子-κB65（NF-κB65）及热休克蛋白70（HSP70）的相关性，探讨Hp相关性胃炎脾胃湿热证的发病机制及灭幽汤治疗慢性胃炎的作用机制。[方法]将70只BALB/c小鼠随机分为对照组、模型组、高浓度灭幽汤组（高灭组）、低浓度灭幽汤组（低灭组）、胃三联组（替硝唑+克拉霉素+枸橼酸铋钾颗粒），每组14只；采用复合因素（肥甘食物+湿热环境+幽门螺杆菌）建立BALB/c小鼠Hp相关性胃炎脾胃湿热证模型；造模成功并连续给药14 d后，分别采用免疫组织化学、Western Blot检测NF-κB65蛋白、HSP70蛋白，qRT-PCR检测NF-κB65 mRNA、HSP70 mRNA的表达情况。[结果]HSP70蛋白及其mRNA的表达：与对照组比较，模型组HSP70蛋白及其mRNA表达均显著增加（P<0.01）；与模型组比较，高灭组、低灭组、胃三联组小鼠HSP70蛋白及其mRNA表达均增加（P<0.01）；高灭组HSP70及其mRNA表达高于低灭组（P<0.05）；胃三联组、高灭组HSP70蛋白及其mRNA表达均增加，但2组间比较差异无统计学意义。NF-κB65蛋白及其mRNA的表达：与对照组比较，模型组NF-κB65蛋白及其mRNA表达增加（P<0.01）；与模型组比较，高灭组、胃三联组小鼠NF-κB65蛋白及其mRNA表达均明显降低（P<0.01）；高灭组NF-κB65蛋白及其mRNA表达低于低灭组（P<0.05）；高灭组、胃三联组NF-κB65蛋白及其mRNA均降低，但2组间比较差异无统计学意义。[结论]灭幽汤可能通过上调HSP70、下调NF-κB65表达而发挥治疗Hp相关性胃炎脾胃湿热证的作用。
- 灭幽汤 /
- Hp相关性胃炎脾胃湿热证 /
- 热休克蛋白70 /
- 核因子-κ /
- B65
Abstract: [Objective] To study the expressions of NF-kappaB65(NF-κB65) and heat shock protein70(HSP 70) in the H.Pylori-sssociated gastritis mice of Pi-Wei hygropyrexia syndrome(PWHS), and to investigate the mechanism of Mieyou Soup in the treatment of chronic gastritis.[Methods] BALB/c mice were randomly divided into five groups:control group, model group, low concentration of Mieyou soup group, high concentration of Mieyou soup group, gastric triad group(Tinidazole, Clarithromycin and Bismuth potassium citrate granules), with 14 rats in each group.Pi-Wei hygropyrexia syndrome mouse model of H.Pylori-associated gastritis was established by composite factor(fatness food+hygropyrexia environment + Helicobacter pylori) After the success of modeling, corresponding treatment was given to mice in each group for 14 days. Immunohistochemistry was used to detect the expression of NF-κB65 protein, western blot to detect the expression of HSP70 protein, and qRT-PCR to detect the expression of NF-κB65 mRNA and HSP70 mRNA.[Results] Compared with control group, the expression of HSP70 protein and HSP70 mRNA in BALB/c mice model group was significantly increased(P<0.01) Compared with the model group, the HSP70 protein and HSP70 mRNA in high and low concentrations of Mieyou soup group as well as the gastric triad group were increased, and the differences were statistically significant(P<0.01) The expression of HSP70 protein and HSP70 mRNA in high-concentration Mieyou soup group was higher than low-concentrations group(P<0.05) The expression of HSP70 protein and HSP70 mRNA in gastric triad group and high-concentration Mieyou soup group were increased, but the difference was insignificant(P>0.05).Compared with control group, the expression of NF-κB65 protein and NF-κB65 mRNA in the model group was significantly increased(P<0.01) Compared with the model group, the NF-κB65 protein and NF-κB65 mRNA in high-concentration Mieyou soup group and gastric triad group were decreased, and the differences were statistically significant(P<0.01) The expression of NF-κB65 protein and NF-κB65 mRNA in high-concentration Mieyou soup was lower than those of low-concentration Mieyou soup group(P<0.05) The expression of NF-κB65 protein and NF-κB65 mRNA in gastric triad group and high-concentration Mieyou soup were decreased, but the difference was not statistically significant(P>0.05).[Conclusion] Mieyou Soup may play a role in the treatment of H.Pylori-associated gastritis mice of Pi-Wei hygropyrexia syndrome by raising the expression of HSP70, reducing the expression of NF-κB65 protein expression.
- Mieyou Soup /
- H.Pylori-associated gastritis mice of Pi-Wei hygropyrexia syndrome /
- HSP70 /
- NF-κ /
- B65

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