The protective effect and mechanism of puerarin on energy metabolism in hepatic reperfusion injury rabbits
-
摘要: [目的]研究葛根素对兔缺血再灌注损伤中肝脏能量代谢的改善作用及其机制。[方法]将30只家兔随机均分为对照组（C组）、缺血/再灌注组（IR组）和葛根素组（Pur组）。测定血浆中谷丙转氨酶（ALT）浓度和肝组织内一磷酸腺苷（AMP）、二磷酸腺苷（ADP）、三磷酸腺苷（ATP）、总腺苷酸量（TAN）、肝脏的细胞能荷（EC）、丙二醛（MDA）、超氧化物歧化酶（SOD）、一氧化氮（NO）、内皮素（ET）、NO/ET、血栓素B2（TXB2）、前列腺素F1α（PGF1α）、TXB2/PGF1α水平，并观察肝细胞形态学的改变。[结果]与C组比较，IR组血浆ALT浓度、肝组织AMP、ADP、MDA、ET含量显著升高（P<0.01），TXB2、TXB2/PGF1α亦明显升高（P<0.05），肝组织ATP、EC、SOD、NO/ET显著下降（P<0.01），NO、PGF1α亦明显降低（P<0.05），TAN差异无统计学意义（P>0.05），肝细胞形态学异常改变；与IR组比较，Pur组血浆ALT浓度、肝组织AMP、MDA、ET含量显著降低（P<0.01），肝组织ADP、TXB2/PGF1α亦明显减少（P<0.05），TXB2略低于IR组（P>0.05），肝组织ATP、EC、SOD、NO/ET显著升高（P<0.01），肝组织NO、PGF1α亦明显增高（P<0.05），TAN差异无统计学意义（P>0.05），肝细胞形态学异常改变较大程度减轻。[结论]葛根素（60 mg/kg）预处理可通过拮抗氧化损伤、调节NO/ET和前列腺素I2/血栓素A2（PGI2/TXA2）失衡而改善肝细胞的能量代谢，有效防治兔肝再灌注损伤。Abstract: [Objective] To observe the protective effect and mechanism of puerarin on energy metabolism in hepatic reperfusion injury rabbits.[Methods] Thirty rabbits were randomly divided into control group (group C), ischemia/reperfusion injury group(group IR) and puerarin group(group Pur).We investigated alanine aminotransferase(ALT) in plasma, adenosine monphosphate(AMP), adenosine diphosphate(ADP), adenosine triphosphate(ATP), total adenylic acid number(TAN), energy charge(EC), malondialdehyde(MDA), superoxide dismutase(SOD), nitric oxide(NO), endothelin(ET), NO/ET, thromboxane B2(TXB2), prostaglandin F1α(PGF1α) and TXB2/PGF1αin liver tissue and morphological changes of hepatic cells.[Results] Compared to group C, ALT in plasma and AMP, ADP, MDA, ET in hepatic tissue were obviously higher(P<0.01), and TXB2, TXB2/PGF1α in group IR were also higher(P<0.05), while ATP, EC, SOD, NO/ET in hepatic tissue were evidently lower(P<0.01), and NO and hepatic PGF1α were lower, too(P<0.05).There was no significant difference showed in hepatic TAN(P>0.05) and there existed obvious morphological abnormality in hepatic cells.Compared to group IR, ALT in plasma and AMP, MDA and ET in hepatic tissue of group Pur were evidently depressed(P<0.01), and ADP and TXB2/PGF1α in hepatic tissue were depressed, too(P<0.05), while hepatic TXB2 was a little lower than group IR(P>0.05), and ATP, EC, SOD, NO/ET in hepatic tissue were significantly elevated(P<0.01), and NO and PGF1α were also elevated(P<0.05).There was no obvious difference in hepatic TAN(P>0.05) and the morphological abnormality in hepatic cells was notably alleviated.[Conclusion] The fact that Pur alleviated the hepatic reperfusion injury suggests that it improve the hepatic energy metabolism via increasing antioxidation ability and regulating the balance of NO/ET and prostaglandin I2/thromboxane A2(PGI2/TXA2).
-
Key words:
- reperfusion injury /
- puerarin /
- traditional Chinese medicine /
- energy metabolism /
- liver
-
-
[1] 沈洁, 刘洋.异氟烷预处理对大鼠肝缺血再灌注损伤的线粒体机制探讨[J].实用药物与临床, 2014, 17(1):1-4.
[2] 许冬武, 王万铁, 徐正祄, 等.葛根素注射液对家兔肝缺血再灌注损伤的防治作用[J].温州医学院学报, 2005, 35(6):440-442.
[3] 王青, 许冬武, 王万铁, 等.葛根素对家兔肝缺血再灌注损伤时血小板聚集功能的影响[J].温州医学院学报, 2006, 36(4):328-329.
[4] 张福志, 石璐, 郝卯林, 等.葛根素对肝缺血-再灌注损伤兔血清IL-1β、TNF-α的影响[J].中医药学报, 2011, 39(2):25-27.
[5] 刘国岩, 荚卫东, 许戈良, 等.肝脏缺血再灌注损伤的机制及防治进展[J].国际外科学杂志, 2013, 40(1):50-53.
[6] CHEN H E, MA Y C, LEI Y, et al.Effects of ginseng polysaccharides on hepatocellular energy metabolism in hepatic ischemia reperfusion injury in rabbits[J].J Liver, 2012, 1(2):1-4.
[7] SENER G, SEHIRLI O, ERCAN F, et al.Protective effect of MESNA (2-mercaptoethane sulfonate) against hepatic ischemia/reperfusion injury in rats[J].Surg Today, 2005, 35(7):575-580.
[8] 王万铁, 林丽娜, 徐正祄, 等.钙通道阻滞剂抗肝缺血-再灌注损伤作用机制的实验研究[J].中国临床药理学与治疗学杂志, 1999, 4(1):33-35.
[9] 王成友, 倪勇, 刘琰, 等.兔肝缺血/再灌注损伤肝组织氧压及线粒体形态观察[J].肝胆外科杂志, 2002, 10(6):470-472.
[10] 王卫, 许冬武, 王万铁, 等.葛根素对家兔肝缺血再灌注一氧化氮、内皮素-1的影响[J].肝胆胰外科杂志, 2006, 18(3):158-160.
[11] 郑艳容, 陈亮, 颜王鑫, 等.人参多糖对兔肝缺血/再灌注损伤时TXB2/PGF1α平衡的调控作用[J].中国临床药理学与治疗学, 2012, 17(5):513-516.
-
计量
- 文章访问数: 249
- PDF下载数: 191
- 施引文献: 0
下载: