Effects of Kangfuxin Liquid on gastric mucosal injury in rats induced by argon plasma coagulation
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摘要: [目的]本实验旨在观察热损伤所致胃人工溃疡的特点及康复新液对氩等离子凝固术诱导的大鼠胃人工溃疡的疗效。[方法]选取SD大鼠50只，雌雄各半，体重约200 g，其中42只采用氩等离子（电凝指数A50，氩气流量2.0 L/min，输出功率60 W）灼烧大鼠胃黏膜造成胃人工溃疡，建立胃人工溃疡大鼠模型，8只仅做手术不灼烧胃黏膜。1周后共存活40只（其中仅做手术不灼烧胃黏膜的存活6只，灼烧胃黏膜的存活34只）。随机选取5只灼烧胃黏膜的大鼠解剖，取出胃组织肉眼观察溃疡形态及病理组织学检测。将剩余35只大鼠分为6组：正常对照组（6只）；模型组（6只）；阳性对照组（6只）；康复新组大剂量组（6只）；康复新中剂量组（6只）；康复新小剂量组（5只）。治疗组给予康复新液灌胃，阳性对照组给予铝碳酸镁片灌胃。治疗1个月后，取各组动物血液及溃疡部位组织，检测血常规、组织病理学检查以及血清EGF、胃组织bFGF表达。[结果]胃溃疡模型中大鼠的胃组织可见灼烧部位胃壁有明显的凹陷、不平整、存在充血和出血的现象，但无穿孔。经康复新液治疗后明显好转，溃疡面缩小，无一例出血和红肿、溃疡面几乎平整。此外，康复新液治疗组较未治疗组，白细胞、红细胞、血小板均具有明显的升高趋势（P<0.05），而苏木精-伊红染色法检测发现，大剂量的康复新液处理胃溃疡的大鼠后，胃组织中炎症细胞浸润减少，炎症反应得到了明显的缓解。正常对照组大鼠EGF、bFGF的表达为弱阳性，溃疡模型组为强阳性表达。康复新液治疗组和西药治疗组均能较正常对照组明显增加大鼠血清中EGF和胃组织bFGF水平，差异具有统计学意义（P<0.05）。[结论]康复新液对大鼠胃人工溃疡有显著疗效，且呈一定剂量相关性。康复新液中、大剂量组对氩等离子诱导的大鼠胃人工溃疡疗效优于铝碳酸镁。康复新液能够通过升高胃组织内bFGF含量来促进氩等离子凝固术诱导的胃人工溃疡的愈合。Abstract: [Objective] To observe the characteristics of the artificial stomach ulcers caused by thermal damage and Kangfuxin Liquid of argon plasma coagulation induced rat gastric ulcer the curative effect of artificial.[Methods] Fifty SD rats were randomly selected, of which 42 with argon plasma (electric coagulation index A50, argon gas flow rate of 2.0 L/min, the output power of 60 w) burn rat gastric mucosa caused by artificial stomach ulcer, establish artificial stomach ulcer model in rats, 8 surgery not only burn gastric mucosa. Coexistence 40 only after 1 week (only for surgery don't burn the survival of the gastric mucosa 6, burning the survival of the gastric mucosa 34 only). Randomly selected five burning anatomy of the gastric mucosa of rats, remove the stomach tissue formation and histopathological examination confirmed that macroscopic observation ulcer ulcer, building success. The experimental group and use:the remaining 35 only rats were divided into 6 groups:normal control group (6); the model groups (6 saline lavage); positive control group (6 hydrotalcite lavage); Kangfuxin Liquid group high-dose group (6); Kangfuxin Liquid dose group (6); Kangfuxin Liquid low-dose group (5).Positive control group were treated with Hydrotalcite Tablets, and treatment groups were given Kangfuxin Liquid.One month later, blood samples were taken for blood routine test, detection of EGF expression, and ulcer tissues of each group were taken for detection of bFGF expression, histopathologic morphology and HE staining.[Results] The burning parts of the stomach in model group were sagged and uneven, with congestion and bleeding, but no perforation. The phenomena were significantly improved after treatment of Kangfuxin Liquid, with no bleeding or swelling, and ulcers almost flat. In addition, compared with the untreated group, Kangfuxin Liquid significantly increased the white blood cells, red blood cells and platelets (P<0.05). HE staining showed that lymphocyte infiltration in stomach tissue reduced after treatment with high-dose Kangfuxin Liquid, and inflammatory response was significantly alleviated. The expression of EGF and bFGF were weak positive in the normal control group and strong positive in model group. Compared with the control group, the level of EGF in serum and bFGF in gastric tissue were significantly increased in the KangFuxin Liquid and western medicine treatment group (P<0.05).[Conclusion] Kangfuxin Liquid of argon plasma coagulation technique of the artificial stomach ulcer of rats induced by significant curative effect, and a certain dose of relevance.Kangfuxin Liquid large dose group of the artificial stomach ulcer of rats induced by argon plasma curative effect is better than that of aluminum and magnesium carbonate. Kangfuxin Liquid through increased gastric tissues bFGF content to promote the argon plasma coagulation technique induced gastric ulcer healing.
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Key words:
- Kangfuxin Liquid /
- gastric mucosal injury /
- growth factor /
- treatment
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[1] BAUD D, WINDRIM R, KACHURA J R, et al.Minimally invasive fetal therapy for hydropic lung masses:three different approaches and review of the literature[J].Ultrasound Obstet Gynecol, 2013, 42:440-448.
[2] BRAUN A, RICHTER-SCHRAG H J, FISCHER A, et al.Minimally invasive therapy of perforations at the esophagogastric junction by over-the-scope clipping[J].Endoscopy, 2013, 45:E133-134.
[3] SACKS D, BLACK C M, COGNARD C, et al.Multisociety consensus quality improvement guidelines for intraarterial catheter-directed treatment of acute ischemic stroke, from the American Society of Neuroradiology, Canadian Interventional Radiology Association, Cardiovascular and Interventional Radiological Society of Europe, Society for Cardiovascular Angiography and Interventions, Society of Interventional Radiology, Society of NeuroInterventional Surgery, European Society of Minimally Invasive Neurological Therapy, and Society of Vascular and Interventional Neurology[J].AJNR Am J Neuroradiol, 2013, 34:E0.
[4] WAHAB P J, MULDER C J, den HARTOG G, et al.Argon plasma coagulation in flexible gastrointestinal endoscopy:pilot experiences[J].Endoscopy, 1997, 29:176-181.
[5] 李树楠, 张华明.美洲大蠊稳定性的实验研究[J].大理医学院学报, 1994, 3(4):9-11.
[6] 李树楠, 李辉, 张华明, 等.美洲大蠊中促进肉芽生长成份的研究[J].云南医药, 1987, 30(3):174-177.
[7] 李树楠.蟑螂之妙用[J].云南中医杂志, 1981, 2(5):25-25.
[8] 王良, 黄秀深, 陈瑾, 等.康复新液促进慢性胃溃疡愈合作用的研究[J].四川中医, 2011, 30(7):33-35.
[9] 陈新, 王洪, 张艳萍.康复新液的最新临床应用[J].中国医药指南, 2008, 6(17):89-96.
[10] 张俊, 孟令贺, 单士军, 等.康复新液对实验性大鼠皮肤切割伤痂下愈合的影响[J].天津医科大学学报, 2014, 20(3):192-195.
[11] 李勇, 吴洪文, 董向军.康复新液治疗胃溃疡的作用机制[J].中国药物与临床, 2008, 8(6):495-496.
[12] 李廷荃, 马贵同, 赵晋明.丹参对大鼠胃溃疡愈合过程中碱性成纤维细胞生长因子及其受体表达的影响[J].上海中医药杂志, 2005, 51(12):51-53.
[13] 王玮, 孙梅.胃肠黏膜抗损伤和修复新进展[J].世界华人消化杂志, 2005, 13(19):2355-2359.
[14] 刘玮, 潮欣宜, 胡焕明, 等.金葵胃药对大鼠胃黏膜保护作用的实验研究[J].中国实验方剂学杂志, 2007, 13(1):39-40.
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