藤龙补中汤对大肠癌肺转移及肿瘤相关巨噬细胞作用

邓珊; 安红梅; 胡兵

doi:10.3969/j.issn.1671-038X.2016.07.07

藤龙补中汤对大肠癌肺转移及肿瘤相关巨噬细胞作用

- 1 上海中医药大学附属龙华医院肿瘤科中医肿瘤研究所, 上海 200032;
- 2 上海中医药大学附属龙华医院科技处, 上海 200032
基金项目:
国家自然科学基金(No:81273726

81473625)

上海市进一步加快中医药事业发展三年行动计划(No:ZY3-CCCX-3-3025)

上海市组织工程重点实验室开放课题(No:TE1401)

详细信息

作者简介:
邓珊,女,硕士研究生,研究方向:中西医结合肿瘤基础与临床

通讯作者: 胡兵,E-mail:beearhu@hotmail.com

中图分类号: R735

收稿日期: 2015-10-19

Teng-Long-Bu-Zhong Decoction inhibits metastasis and tumor-associated macrophages in colorectal carcinoma

- 1 Department of Oncology, Institute of Traditional Chinese Medicine in Oncology, Shanghai 200032, China;
- 2 Department of Science & Technology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China

More Information

Corresponding author: HU Bing, E-mail: beearhu@hotmail.com

Received Date: 19 October 2015

摘要

摘要: [目的]观察藤龙补中汤对大肠癌肺转移和肿瘤相关巨噬细胞(II型巨噬细胞)的作用。[方法]采用尾静脉注射法建立小鼠大肠癌肺转移模型,随机分为3组,每组10只;对照组蒸馏水灌胃,每天1次;中药组,藤龙补中汤灌胃,每天1次;化疗组,5-Fu腹腔注射,每周1次;连续用药3周后,剥离小鼠肺组织,称重并计数转移结节;免疫组织化学方法检测小鼠肺转移灶
- 大肠癌 /
- 肺转移 /
- 藤龙补中汤 /
- 肿瘤相关巨噬细胞 /
- VEGF /
- 血管生成
Abstract: [Objective] To observe the effects of Teng-Long-Bu-Zhong Decoction (TLBZD),a traditional Chinese herbal formula, on lung metastasis and tumor-associated macrophages (TAM) (Type II macrophage) in colorectal carcinoma. [Methods] The model of lung metastasis of colorectal carcinoma in mice was established by tail vein injection. Those mice were randomly divided into three groups (10 mice per group): the control group was intragastric administrated with distilled water once a day; the Chinese medicine group was intragastric administrated with TLBZD daily; and chemotherapy group were administrated with 5-Fu by intraperitoneal injection once a week. After three weeks of treatment, the mouse lungs were stripped and weighed, and the metastatic nodules in lung surface were counted. The expression of Arginase I (the marker gene of tumor associated macrophages), VEGF and angiogenesis in metastatic lesions were detected by immunohistochemistry method.[Results] Compared with control group, TLBZD significantly inhibited lung metastasis of colorectal carcinoma. After three weeks of TLBZD treatment, the lung weight and metastatic nodules were significantly reduced. TLBZD also significantly decreased TAM, and reduced the expression of VEGF and angiogenesis.[Conclusion] TLBZD can inhibit lung metastasis of colorectal carcinoma, and it may be related to inhibition of TAM and angiogenesis.
- colorectal carcinoma /
- lung metastasis /
- Teng-Long-Bu-Zhong Decoction /
- tumor-associated macrophages /
- VEGF /
- angiogenesis

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