Effect of Zhizhu Kuanzhong capsule on the improvement of micro-inflammation in the duodenum of rats with functional dyspepsia
-
摘要: 目的 探讨枳术宽中胶囊对功能性消化不良(functional dyspepsia，FD)大鼠十二指肠微炎症的干预效应。 方法 将36只SD大鼠随机分为空白组与造模组，通过复合造模法(碘乙酰胺灌胃+小平台站立)建立FD大鼠。将成功建模的FD大鼠随机分为模型组、莫沙必利组和低、中、高剂量枳术宽中胶囊组。给药2周后，观察各组大鼠一般状况、检测各组大鼠体质量及胃排空率，采用酶联免疫吸附试验法测定各组大鼠血清中IL-1β、IL-18的含量，以蛋白质印迹法检测NF-κB蛋白的表达水平。 结果 与模型组比较，各剂量枳术宽中胶囊组与莫沙必利组大鼠经药物干预后毛发逐渐整洁光亮，自主活动频率增加，反应速度加快，粪便恢复正常，且体质量均呈上升状态(P＜0.05)。苏木精-伊红染色显示，各组大鼠胃组织形态结构完整，细胞排列整齐。与模型组比较，各剂量枳术宽中胶囊组与莫沙必利组大鼠十二指肠苏木精-伊红染色肠绒毛排列整齐，炎症细胞浸润减少，细胞间隙较清晰。与模型组比较，中高剂量枳术宽中胶囊组与莫沙必利组大鼠血清IL-18及IL-1β含量明显降低(P＜0.05)，且中高剂量枳术宽中胶囊组与莫沙必利组大鼠十二指肠组织中的NF-κB蛋白表达亦明显降低(P＜0.05)。 结论 枳术宽中胶囊可以有效抑制十二指肠微炎症。Abstract: Objective To investigate the interventional effect of Zhizhu Kuanzhong capsule(ZZKZ) on duodenal microinflammation in rats with functional dyspepsia(FD). Methods Thirty-six SD rats were randomly divided into the normal group and the model group, and the FD rats were established by the composite modeling method(iodoacetamide gavage and small platform standing). The successfully modeled FD rats were randomly divided into the model group, mosapride group and low, medium and high dose ZZKZ group. After 2 weeks of drug administration, the general condition of rats in each group was observed, the body mass and gastric emptying rate of rats in each group were detected. The levels of IL-1β and IL-18 in the serum of each group were determined using an enzyme-linked immunosorbent assay, and the expression levels of NF-κB proteins were detected by Western blotting. Results Compared with the model group, rats in ZZKZ group and the mosapride group showed improvements after drug intervention. Their fur became gradually clean and shiny, spontaneous activity frequency increased, response speed quickened, stool returned to normal, and body weight showed an upward trend(P < 0.05). Hematoxylin-eosin staining revealed that the gastric tissue structure of rats in all groups remained intact, with cells arranged neatly. Compared with the model group, rats in ZZKZ group and the mosapride group had duodenal villi arranged neatly, with reduced infiltration of inflammatory cells and clearer intercellular spaces in hematoxylin-eosin stained duodenal tissue. Compared with the model group, the serum levels of IL-18 and IL-1β in the medium and high dose ZZKZ group and mosapride group were reduced(P < 0.05). The expression of NF-κB protein in duodenal tissues was reduced in both the medium and high dose ZZKZ group and the mosapride group compared with the model group(P < 0.05). Conclusion Zhizhu Kuanzhong capsule can effectively inhibit duodenal microinflammation.
-
Key words:
- Zhizhu Kuanzhong capsule /
- functional dyspepsia /
- duodenum /
- microinflammation /
- rats
-
-
表 1 各组大鼠体质量及胃排空率比较
X±S 组别 鼠数 体质量/g 胃排空率/% 空白组 6 415.33±42.362) 71.28±8.332) 模型组 6 223.25±25.54 45.39±5.25 低剂量枳术宽中胶囊组 6 280.17±33.611) 55.89±7.861) 中剂量枳术宽中胶囊组 6 374.08±56.022) 66.83±5.352) 高剂量枳术宽中胶囊组 6 391.50±15.972) 68.94±8.822) 莫沙必利组 6 365.58±47.322) 70.56±8.922) 与模型组比较，1)P＜0.05，2)P＜0.01。 
下载: 导出CSV
表 2 各组大鼠血清IL-18及IL-1β含量比较
X±S 组别 IL-18/(pg/mL) IL-1β/(pg/mL) 空白组 35.27±18.711) 5.26±1.251) 模型组 71.66±9.74 18.05±12.33 低剂量枳术宽中胶囊组 26.06±13.892) 11.86±7.07 中剂量枳术宽中胶囊组 34.61±22.062) 5.99±0.241) 高剂量枳术宽中胶囊组 28.64±22.061) 7.30±1.701) 莫沙必利组 30.98±15.382) 3.43±1.841) 与模型组比较，1)P＜0.05，2)P＜0.01。
下载: 导出CSV
-
[1] Ford AC, Mahadeva S, Carbone MF, et al. Functional dyspepsia[J]. Lancet, 2020, 396(10263): 1689-1702. doi: 10.1016/S0140-6736(20)30469-4
[2] Sperber AD, Bangdiwala SI, Drossman DA, et al. Worldwide Prevalence and Burden of Functional Gastrointestinal Disorders, Results of Rome Foundation Global Study[J]. Gastroenterology, 2021, 160(1): 99-114. doi: 10.1053/j.gastro.2020.04.014
[3] Vanuytsel T. Novel insights in the pathophysiology and management of functional dyspepsia[J]. Acta Gastro Enterologica Belgica, 2023, 86(1): 68-73. doi: 10.51821/86.1.11629
[4] 李牡丹, 包永欣. 基于肠上皮黏膜屏障探讨功能性消化不良发病机制研究[J]. 辽宁中医药大学学报, 2023, 25(5): 175-179.
[5] Wauters L, Ceulemans M, Schol J, et al. The Role of Leaky Gut in Functional Dyspepsia[J]. Front Neurosci, 2022, 16: 851012. doi: 10.3389/fnins.2022.851012
[6] Wang J, Zhao H, Lv K, et al. Pterostilbene Ameliorates DSS-Induced Intestinal Epithelial Barrier Loss in Mice via Suppression of the NF-kappaB-Mediated MLCK-MLC Signaling Pathway[J]. J Agric Food Chem, 2021, 69(13): 3871-3878. doi: 10.1021/acs.jafc.1c00274
[7] 王晓彤, 董佳梓, 王建波, 等. 电针调节TLR4、NLRP3改善功能性消化不良大鼠胃黏膜炎症反应机制研究[J]. 辽宁中医药大学学报, 2023, 25(2): 109-113.
[8] 曾毅. 电针对FD模型大鼠mTOR信号通路调节Ghrelin机制研究[D]. 武汉: 湖北中医药大学, 2021.
[9] 刘梦雅, 成映霞, 白敏, 等. 香砂六君子汤对脾胃虚弱型功能性消化不良大鼠的干预机制研究[J]. 中国实验动物学报, 2023, 31(2): 232-239.
[10] 李娟娟, 王凤云, 吕林, 等. 香砂六君子汤对功能性消化不良脾虚证大鼠胃动力及CRF, UCN2表达的影响[J]. 中国实验方剂学杂志, 2022, 28(3): 1-7.
[11] 常雨, 张艮霜, 张艺川, 等. 中医药治疗功能性消化不良相关信号通路的研究进展[J]. 中国中药杂志, 2023, 48(20): 5397-5403.
[12] 项红霞, 王岩, 冷静, 等. 枳术宽中胶囊联合维酶素片治疗慢性萎缩性胃炎的临床研究[J]. 现代药物与临床, 2020, 35(4): 668-672.
[13] 杨斌. 基于网络药理学探讨枳术宽中胶囊治疗功能性消化不良的作用机制及分子对接验证[D]. 太原: 山西医科大学, 2022.
[14] 刘晶, 李峰, 唐旭东, 等. 功能性消化不良脾虚证动物模型的制作及评价[J]. 环球中医药, 2015, 8(6): 701-705.
[15] 赵鲁卿, 时昭红, 张声生. 功能性消化不良中医诊疗专家共识意见(2023)[J]. 中华中医药杂志, 2024, 39(3): 1372-1378.
[16] 黄菊萍, 谭禾英, 孙姚, 等. 疏肝解郁消痞方对肝胃不和型功能性消化不良患者的临床疗效[J]. 中成药, 2023, 45(7): 2202-2205.
[17] 陈欢, 宁博, 张洁, 等. 中药治疗功能性消化不良系统评价再评价[J]. 国际中医中药杂志, 2023, 45(7): 898-906.
[18] 刘洪武, 谢宜春, 黎丽群, 等. 中药汤剂治疗功能性消化不良系统评价的AMSTAR2质量评价及GRADE证据分级[J]. 中国中医药信息杂志, 2020, 27(4): 98-103.
[19] 谢宜春, 刘洪武, 黎丽群, 等. 中成药治疗功能性消化不良的系统评价再评价[J]. 广西医学, 2019, 41(18): 2344-2349.
[20] Xiao Y, Li Y, Shu J, et al. The efficacy of oral Zhizhu Kuanzhong, a traditional Chinese medicine, in patients with postprandial distress syndrome[J]. J Gastroenterol Hepatol, 2019, 34(3): 526-531.
[21] 贺琼. 枳术宽中胶囊联合莫沙比利治疗功能性消化不良临床研究[J]. 陕西中医, 2017, 38(4): 471-472.
[22] 秦波, 张俊. 枳术宽中胶囊对功能性消化不良并抑郁患者的影响[J]. 中国实验方剂学杂志, 2015, 21(8): 186-189.
[23] Xiao Z, Xu J, Tan J, et al. Zhizhu Kuanzhong, a traditional Chinese medicine, alleviates gastric hypersensitivity and motor dysfunction on a rat model of functional dyspepsia[J]. Front Pharmacol, 2022, 13: 1026660.
[24] Wauters L, Ceulemans M, Frings D, et al. Proton Pump Inhibitors Reduce Duodenal Eosinophilia, Mast Cells, and Permeability in Patients With Functional Dyspepsia[J]. Gastroenterology, 2021, 160(5): 1521-1531.
[25] Giuliani C, Bucci I, Napolitano G. The Role of the Transcription Factor Nuclear Factor-kappa B in Thyroid Autoimmunity and Cancer[J]. Front Endocrinol(Lausanne), 2018, 9: 471.
[26] Zinatizadeh MR, Schock B, Chalbatani GM, et al. The Nuclear Factor Kappa B(NF-kB)signaling in cancer development and immune diseases[J]. Genes Dis, 2021, 8(3): 287-297.
[27] 孙婷, 陈泉, 肖继, 等. NF-κB亚基泛素化研究进展[J]. 生理科学进展, 2018, 49(3): 217-221.
-
计量
- 文章访问数: 128
- 施引文献: 0