藤龙补中汤对大肠癌LoVo细胞失巢凋亡的作用及机制

王双双; 郑佳露; 陈锦芳; 邬世威; 师子曼; 胡兵

doi:10.3969/j.issn.1671-038X.2023.09.06

藤龙补中汤对大肠癌LoVo细胞失巢凋亡的作用及机制

- 1.
  上海中医药大学附属龙华医院中医肿瘤研究所(上海，200032)
- 2.
  上海中医药大学附属龙华医院肿瘤科
基金项目:
国家自然科学基金(No：82074352)；上海市自然科学基金(No：20ZR1458700)

详细信息

通讯作者: 胡兵, E-mail: beearhu@shutcm.edu.cn

中图分类号: R735.34

收稿日期: 2023-01-02

刊出日期: 2023-09-15

Effects and mechanism of Tenglong Buzhong Decoction on anoikis of LoVo colorectal cancer cells

- 1.
  Institute of Traditional Chinese Medicine in Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China
- 2.
  Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine

More Information

Corresponding author: HU Bing, E-mail: beearhu@shutcm.edu.cn

Received Date: 02 January 2023

Publish Date: 15 September 2023

摘要

摘要: 目的观察藤龙补中汤对人大肠癌LoVo细胞失巢凋亡的作用及机制。方法将藤龙补中汤作用于悬浮生长的LoVo细胞，CCK-8试剂检测细胞增殖；试剂盒检测失巢凋亡、Caspases活性和活性氧；Western blot检测蛋白表达和磷酸化。结果藤龙补中汤可以抑制LoVo细胞悬浮生长，诱导失巢凋亡，活化Caspase-3、Caspase-8和Caspase-9。Caspases抑制剂Z-VAD-FMK可拮抗藤龙补中汤对失巢凋亡的作用。藤龙补中汤可以上调活性氧生成，活性氧清除剂N-乙酰-L-半胱氨酸可拮抗藤龙补中汤对Caspases的作用。藤龙补中汤还可降低整合素αV和β3的表达，抑制黏着斑激酶的磷酸化。结论藤龙补中汤可诱导LoVo细胞失巢凋亡，其机制可能与上调活性氧、下调整合素αV和β3的表达、抑制黏着斑激酶的磷酸化相关。
- 大肠癌 /
- 藤龙补中汤 /
- 悬浮生长 /
- 失巢凋亡 /
- Caspases /
- 活性氧 /
- 整合素αV /
- 整合素β3 /
- 黏着斑激酶
Abstract: Objective To study the effects and mechanism of Tenglong Buzhong Decoction(TBD) on anoikis in human colorectal cancer LoVo cells.Methods Suspension grown LoVo cells were treated with TBD. Cell proliferation was detected by cell counting kit-8(CCK-8) assay. Anoikis, Caspases activaties and reactive oxygen species(ROS) were measured by commercial kit. Proteins expression and phosphorylation were identified by Western blot.Results TBD inhibited suspension growth of LoVo cells, induced anoikis and activated Caspase-3, Caspase-8, and Caspase-9. Caspases inhibitor Z-VAD-FMK antagonized the effects of TBD on anoikis. TBD up-regulated ROS, ROS scavenger N-acetyl-L-cysteine(NAC) attenuated the effects of TBD on Caspases. TBD also inhibited the expressions of integrin aV and β3, and phosphorylation of focal adhesion kinase(FAK).Conclusion TBD induces anoikis in LoVo cells, and may be related to up-regulation of ROS, and inhibition of integrin aV and integrin β3 expressions, and FAK phosphorylation.
- colorectal cancer /
- Tenglong Buzhong Decoction /
- suspension growth /
- anoikis /
- Caspases /
- reactive oxygen species /
- integrin aV /
- integrin β3 /
- focal adhesion kinase

HTML全文

图 1 TBD对LoVo细胞悬浮生长的影响

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图 2 TBD对失巢凋亡的影响

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图 3 TBD对Caspases活性的影响

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图 4 TBD对活性氧的影响

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图 5 TBD对整合素-FAK信号转导的影响

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