清肠温中方对溃疡性结肠炎患者黏膜屏障功能的调控作用研究

韩啸; 李军祥; 陈晓伟; 王志斌; 袁亚利; 邢韵淇; 张文基; 王宇轩; 刘豫玥

doi:10.3969/j.issn.1671-038X.2022.10.06

清肠温中方对溃疡性结肠炎患者黏膜屏障功能的调控作用研究

- 1.
  北京中医药大学(北京，100029)
- 2.
  北京中医药大学东方医院
基金项目:
中医药传承与创新“百千万”人才工程——岐黄学者(李军祥)[No：国中医药人教函(2018)284号]；北京中医药大学青年教师项目(No：2019-JYB-JS-119)

详细信息

通讯作者: 刘豫玥，E-mail：liuyuyue1985@126.com

中图分类号: R574.62

收稿日期: 2022-08-14

刊出日期: 2022-10-15

Regulatory effect of Qingchang Wenzhong Decoction on mucosal barrier function in patients with ulcerative colitis

- 1.
  Beijing University of Chinese Medicine, Beijing, 100029, China
- 2.
  Dongfang Hospital, Beijing University of Chinese Medicine

More Information

Corresponding author: LIU Yuyue, E-mail: liuyuyue1985@126.com

Received Date: 14 August 2022

Publish Date: 15 October 2022

摘要

摘要: 目的探讨清肠温中方对溃疡性结肠炎患者肠黏膜屏障及信号转导的影响。方法回顾性分析56例轻中度溃疡性结肠炎患者的资料，病例来源于北京中医药大学东方医院消化内科门诊及病房，全部给予清肠温中方配方颗粒治疗8周。记录治疗前后改良Mayo评分，同时取病变肠段肠黏膜和正常肠段肠黏膜进行苏木精-伊红染色评价病理组织学改变，PAS染色评估结肠杯状细胞数量，免疫组织化学染色检测转录激活蛋白6(STAT6)及黏蛋白2(MUC2)的表达。结果清肠温中方治疗能显著降低轻中度溃疡性结肠炎患者的改良Mayo评分，改善其肠黏膜的病理损伤和炎症细胞浸润(P＜0.01)，增加结肠杯状细胞数量(P＜0.01)，提高结肠黏膜组织黏蛋白MUC2的表达(P＜0.01)，抑制STAT6的表达(P＜0.01)。结论清肠温中方治疗轻中度活动期UC患者临床疗效确切，其作用机制与增强肠道屏障功能有关。
- 溃疡性结肠炎 /
- 清肠温中方 /
- 黏膜屏障功能 /
- 转录激活蛋白6 /
- 黏蛋白2
Abstract: Objective To investigate the effect of Qingchang Wenzhong Decoction(QCWZD) on intestinal mucosal barrier and signal transducer in patients with ulcerative colitis(UC).Methods A retrospective study was conducted to analyze 56 patients with mild to moderate UC who were hospitalized in Department of Gastroenterology, Dongfang Hospital, Beijing University of Traditional Chinese Medicine. All of them were treated with QCWZD granules for 8 weeks. The modified Mayo score before and after treatment was recorded, and normal mucosa and inflammatory mucosa were separated for HE staining and periodic acid Schiff staining. Immunohistochemical staining was used to detect the expression of mucin 2(MUC2) and STAT6.Results QCWZD treatment could significantly reduce the modified Mayo score, improve the pathological damage and inflammatory cell infiltration of intestinal mucosa in patients with mild to moderate UC(P < 0.01), increase the number of colonic goblet cells(P < 0.01), increase the expression of MUC2 in colonic epithelial tissue(P < 0.01), and inhibit the expression of STAT6(P < 0.01).Conclusion QCWZD has a definite clinical effect on patients with mild to moderate active UC, and its mechanism is related to the enhancement of intestinal barrier function.
- ulcerative colitis /
- Qingchang Wenzhong Decoction /
- mucosal barrier function /
- signal transducer and activator of transcription 6 /
- mucin 2

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图 1 清肠温中方对UC患者结肠黏膜组织病理损伤的修复作用

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图 2 清肠温中方对UC患者结肠黏膜杯状细胞的影响

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图 3 清肠温中方对UC患者结肠黏膜组织MUC2表达的影响

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图 4 清肠温中方对UC患者结肠黏膜组织STAT6表达的影响

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