白花蛇舌草干预大肠腺瘤的网络药理学研究

鲁仕昱; 林辉

doi:10.3969/j.issn.1671-038X.2021.05.10

白花蛇舌草干预大肠腺瘤的网络药理学研究

鲁仕昱,
林辉^,

- 上海中医药大学附属普陀医院消化内科(上海, 200062)
基金项目:
上海中医药大学附属普陀医院科研课题计划(No:2017316A)

详细信息

通讯作者: 林辉,E-mail:niliuh@163.com

中图分类号: R735.3

收稿日期: 2020-09-14

The network pharmacology study of Hedyotis diffusa on colorectal adenoma

LU Shi-yu,
LIN Hui^,

- Department of Gastroenterology, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China

More Information

Corresponding author: LIN Hui, E-mail: niliuh@163.com

Received Date: 14 September 2020

摘要

摘要: 目的:利用网络药理学探讨白花蛇舌草防治大肠腺瘤的作用靶点、生物过程及相关通路,结合中医学的认识,进一步探讨其干预癌前病变的可行性及潜在机制。方法:在Gene Cards和OMIM数据库获取大肠腺瘤疾病的靶点,中药系统药理学数据库(TCMSP)中检索白花蛇舌草的成分并获取靶点信息,用Cytoscape 3.8.0软件对白花蛇舌草-大肠腺瘤可视化构图,并构建蛋白质相互作用网络。对白花蛇舌草-大肠腺瘤作用靶点进行GO富集分析、蛋白质相互作用网络、KEGG通路富集分析。结果:白花蛇舌草防治大肠腺瘤的作用机制中,可能主要以熊果酸、槲皮素成分为主作用腺瘤,通过EGFR、GRB2、MYC、NTRK1、TP53等靶点参与凋亡信号通路、细胞毒性作用、细胞因子介导的信号通路等生物过程,和激酶结合、转录因子结合、蛋白激酶活性、激酶调节活性等生物功能有关,包含转录因子复合物、Bcl-2家族蛋白、囊泡腔、细胞周期素依赖性蛋白激酶全酶复合物等分子组分。主要通过癌症通路中PI3K-Akt信号通路、MAPK信号通路、癌症中的蛋白多糖等多条通路。结论:在预测白花蛇舌草防治大肠腺潜在机制中,多个成分、靶点、通路作用于疾病,可能对大肠癌发挥预防作用,为进一步实验研究及临床干预早期癌变提供理论基础和参考。
- 网络药理学 /
- 白花蛇舌草 /
- 大肠腺瘤 /
- 作用机制 /
- 中药
Abstract: Objective: This study uses network pharmacology to explore the targets, biological processes and related pathways of Hedyotis diffusa in the prevention and treatment of colorectal adenoma, combined with the knowledge of Traditional Chinese Medicine, to further explore the feasibility and potential mechanism of its intervention in precancerous lesions.Methods: The target of colorectal adenoma disease was obtained from Gene Cards and OMIM databases, the components of Oldenlandia diffusa were retrieved from the Traditional Chinese Medicine System Pharmacology Database(TCMSP) and the target information was obtained, and the Cytoscape 3.8. 0 software was used to analyze Oldenlandia diffusa-large intestine Visualize the adenoma and construct the PPI network. The target of Hedyotis diffusa-colorectal adenoma was analyzed by GO enrichment analysis, protein-protein interaction enrichment analysis, and KEGG pathway enrichment analysis.Results: In the mechanism of Hedyotis diffusa against colorectal adenomas, Ursolic Acid and Quercetin may be the main components of adenomas, through EGFR,GRB2,MYC,NTRK1,TP53, etc.The target is involved in the apoptosis signaling pathway, response to toxic substance, cytokine-mediated signaling pathway, etc. Biological processes are related to biological functions such as kinase binding, transcription factor binding, protein kinase activity, kinase regulatory activity, including transcription factor complexes, Bcl-2 family proteins, vesicle lumen, and cyclin-dependent protein kinases Molecular components such as cyclin dependent protein kinase holoenzyme complex. Mainly through the PI3 K-Akt signaling pathway, MAPK signaling pathway, and Proteoglycans in cancer in cancer pathways.Conclusion: In predicting the potential mechanism of Hedyotis diffusa against colorectal glands, multiple components, targets, and pathways act on the disease, and may play a preventive effect on colorectal cancer, providing a theoretical basis and reference for further experimental research and clinical intervention in early cancer.
- network pharmacology /
- Hedyotis diffusa /
- colorectal adenoma /
- mechanism of action /
- Traditional Chinese Medicine

HTML全文

参考文献(28)

[1]	张晓翠.白花蛇舌草的研究概况[J].时珍国药研究,1998,9(1):92-93.
[2]	南京中医药大学,赵国平,戴慎等.中药大辞典[M].上海:上海科学技术出版社,2006.
[3]	Qari YA.Clinicopathological characterization of colonic polyps[J].Nigerian J Clin Pract,2020,23(8):1048-1053.
[4]	Kim M,Vogtmann E,Ahlquist DA,et al.Fecal Metabolomic Signatures in Colorectal Adenoma Patients Are Associated with Gut Microbiota and Early Events of Colorectal Cancer Pathogenesis[J].mBio,2020,11(1):e03186-19.
[5]	Zhao XX,Liu MH,Wang RL,et al.Effect of Gender and Age on the Correlation between Helicobacter pylori and Colorectal Adenomatous Polyps in a Chinese Urban Population:A Single Center Study[J].Gastroenterol Res Pract,2020,2020:8596038.
[6]	Ze EY,Kim BJ,Jun DH,et al.The Fatty Liver Index:A Simple and Accurate Predictor of Colorectal Adenoma in an Average-Risk Population[J].Dis Colon Rectum,2018,61(1):36-42.
[7]	张荣,林辉.2015年内镜下结肠直肠息肉切除术相关指南与共识解读[J].世界临床药物,2015,36(12):814-819.
[8]	Shi X,Yang Z,Wu Q,et al.Colorectal adenoma recurrence rates among post-polypectomy patients in the placebo-controlled groups of randomized clinical trials:a meta-analysis[J].Oncotarget,2017,8(37):62371-62381.
[9]	Snover DC.Update on the serrated pathway to colorectal carcinoma[J].Human Pathol,2011,42(1):1-10.
[10]	Nguyen LH,Goel A,Chung DC.Pathways of Colorectal Carcinogenesis[J].Gastroenterology,2020,158(2):291-302.
[11]	邱静,钱素婷,曹泽鹏,等.大肠湿热型结直肠腺瘤肠道菌群分布规律研究[J].中国中西医结合消化杂志,2021,29(3):187-192.
[12]	Zhou Q,Chen YG,Xiao J,et al.Traditional Chinese medicine(Xiaoai Jiedu Decoction)as an adjuvant treatment for prevention new colorectal adenomatous polyp occurrence in post-polypectomy:Study protocol for a randomized controlled trial[J].Medicine(Baltimore),2019,98(31):e16680.
[13]	郭秀清.中西医结合治疗大肠多发息肉临床研究[J].中国保健营养,2016,26(8):108-109.
[14]	张北平.一种防治大肠息肉的中药组合物:中国,CN201610117536.0[P].2019-10-01.
[15]	金健,张相安,郭海霞,等.张东岳教授运用消息灵方治疗大肠息肉经验[J].中医研究,2020,33(5):39-41.
[16]	王少墨,王秀薇,姚怡,等.王庆其治疗大肠息肉经验[J].中医杂志,2016,57(15):1278-1280.
[17]	Zhang P,Zhang B,Gu J,et al.The Study of the Effect of Hedyotis diffusa on the Proliferation and the Apoptosis of the Cervical Tumor in Nude Mouse Model[J].Cell Biochemistry Biophy,2015,72(3):783-789.
[18]	Lin L,Cheng K,He Z,et al.A polysaccharide from Hedyotis diffusa interrupts metastatic potential of lung adenocarcinoma A549 cells by inhibiting EMT via EGFR/Akt/ERK signaling pathways[J].Intern J Boil Macromolecul,2019,129:706-714.
[19]	Jia X,Wen Z,Sun Q,et al.Apatinib suppresses the Proliferation and Apoptosis of Gastric Cancer Cells via the PI3K/Akt Signaling Pathway[J].J Balkan Union Oncol,2019,24(5):1985-1991.
[20]	Asati V,Mahapatra DK,Bharti SK.PI3K/Akt/mTOR and Ras/Raf/MEK/ERK signaling pathways inhibitors as anticancer agents:Structural and pharmacological perspectives[J].Eur J Med Chemistry,2016,109:314-341.
[21]	Sun Y,Liu WZ,Liu T,et al.Signaling pathway of MAPK/ERK in cell proliferation,differentiation,migration,senescence and apoptosis[J].J Receptor Signal Transduction Res,2015,35(6):600-604.
[22]	Fang JY,Richardson BC.The MAPK signalling pathways and colorectal cancer[J].Lancet Oncol,2005,6(5):322-327.
[23]	Rauf A,Imran M,Khan IA,et al.Anticancer potential of quercetin:A comprehensive review[J].Phytother Res,2018,32(11):2109-2130.
[24]	Chan EWC,Soon CY,Tan JBL,et al.Ursolic acid:An overview on its cytotoxic activities against breast and colorectal cancer cells[J].J Integrate Med,2019,17(3):155-160.
[25]	Nouri Z,Fakhri S,Nouri K,et al.Targeting Multiple Signaling Pathways in Cancer:The Rutin Therapeutic Approach[J].Cancers,2020,12(8):2276.
[26]	He J,Lu X,Wei T,et al.Asperuloside and Asperulosidic Acid Exert an Anti-Inflammatory Effect via Suppression of the NF-κB and MAPK Signaling Pathways in LPS-Induced RAW 264.7 Macrophages[J].Intern J Molecul,2018,19(7):2027.
[27]	Wang C,Xin P,Wang Y,et al.Iridoids and sfingolipids from Hedyotis diffusa[J].Fitoterapia,2018,124:152-159.
[28]	Li H,Lai Z,Yang H,et al.Hedyotis diffusa Willd.inhibits VEGFCmediated lymphangiogenesis in colorectal cancer via multiple signaling pathways[J].Oncol Rep,2019,42(3):1225-1236.