猪苓多糖对胃肠上皮化生细胞模型的影响

林贞妍; 冯小可; 崔国良; 魏睦新

doi:10.3969/j.issn.1671-038X.2021.01.02

猪苓多糖对胃肠上皮化生细胞模型的影响

- 南京医科大学第一附属医院中医科南京医科大学中西医结合研究所(南京, 210029)
基金项目:
江苏省中医药局科技项目(No:YB2015166)

详细信息

通讯作者: 魏睦新,E-mail:weimuxin@njmu.edu.cn

中图分类号: R445

收稿日期: 2020-09-03

Effect of polyporus umbellatus polysaccharides on gastrointestinal metaplasia cell model induced in vitro

- Department of Traditional Chinese Medicine, the First Affiliated Hospital of Nanjing Medical University, Institute of Integrative Medicine, Nanjing Medical University, Nanjing, 210029, China

More Information

Corresponding author: WEI Muxin, E-mail: weimuxin@njmu.edu.cn

Received Date: 03 September 2020

摘要

摘要: 目的:探讨猪苓多糖对鹅去氧胆酸(CDCA)诱导GES-1细胞株的肠上皮化生相关分子标志物的影响。方法:CCK8法检测不同浓度CDCA、猪苓多糖对GES-1细胞增殖能力的影响;RT-qPCR、Western blot法检测肠上皮化生相关分子标志物表达水平。结果:CDCA诱导后,GES-1细胞的尾型同源盒2(CDX2)、Kruppel样转录因子4(KLF4)蛋白及mRNA表达水平明显上调(P<0.05),性别决定区Y框蛋白2(SOX2)蛋白及mRNA表达水平显著下调(P<0.05)。猪苓多糖干预后可抑制由CDCA诱导的肠上皮化生细胞模型CDX2、KLF4的mRNA和蛋白表达(P<0.05),并促进SOX2 mRNA和蛋白的表达(P<0.05)。结论:猪苓多糖可通过下调CDX2和KLF4的表达,上调SOX2的表达,从而在一定程度上阻断胃黏膜上皮细胞向肠上皮化生转变的进程。
- 胃癌前病变 /
- 胃肠上皮化生 /
- 猪苓多糖 /
- 鹅去氧胆酸 /
- 细胞模型
Abstract: Objective: To explore the effect of polyporus umbellatus polysaccharides on gastrointestinal metaplasia of GES-1 cells induced by chenodeoxycholic acid.Methods: The effects of chenodeoxycholic acid and Polyporus umbellatus polysaccharide at different concentrations on the proliferation of GES-1 cells was detected by CCK8, and the expression of intestinal metaplasia related molecular markers was determined by RT-qPCR and Western blot.Results: After GES-1 cells were induced by chenodeoxycholic acid, the expression levels of CDX2, KLF4 protein and mRNA were significantly up-regulated(P<0.05), while the expression levels of SOX2 protein and mRNA were significantly down-regulated(P<0.05). Polyporus umbellatus polysaccharides can inhibit the expression of mRNA and protein of CDX2 and KLF4 in intestinal metaplasia cell model induced by CDCA(P<0.05), and promote the expression of SOX2 mRNA and protein(P<0.05).Conclusion: Polyporus umbellatus polysaccharides can block the transformation of gastric mucosal epithelial cells to intestinal metaplasia to some extent by down-regulating the expression of CDX2 and KLF4 and up-regulating the expression of SOX2.
- precancerous lesions of gastric carcinoma /
- intestinal metaplasia /
- polyporus umbellalus polysaccharides /
- chenodeoxycholic acid /
- cell model

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