奥曲肽对人肝纤维化进程的调控及相关分子机制研究

蒋亚玲; 周贤; 刘蔚; 杨伟兴; 冯雯; 葛英

doi:10.3969/j.issn.1671-038X.2017.09.04

奥曲肽对人肝纤维化进程的调控及相关分子机制研究

- 1. 西南医科大学附属中医院脾胃科, 四川泸州, 646000;
- 2. 西南医科大学附属医院消化内科, 四川泸州, 646000;
- 3. 绵阳市人民医院神经内科, 四川绵阳 621000
基金项目:
泸州市科技局计划项目 (No:2011-I-S37)

详细信息

作者简介:
蒋亚玲,硕士,主要从事肝纤维化的基础及临床研究

通讯作者: 周贤,E-Mail:zhouxian68@163.com

中图分类号: R575

收稿日期: 2017-05-10

Study on the regulation and molecular mechanism of octreotide on human liver fibrosis

- 1. Department of Gastroenterology, the Affiliated Traditional Chinese Medicine Hospital of Xinan Medical University, Luzhou 646000, China;
- 2. Department of Gastroenterology, the Affiliated Hospital of Xinan Medical University, Luzhou 646000, China;
- 3. Department of Neurology, People's Hospital in Mianyang, Mianyang 621000, China

More Information

Corresponding author: ZHOU Xian, E-mail: zhouxian68@163.com

Received Date: 10 May 2017

摘要

摘要: [目的]:通过探讨奥曲肽干预人肝星状细胞株LX-2后的细胞增殖和凋亡情况以及STAT3、SOCS3的表达水平,进一步研究奥曲肽对人肝纤维化进程可能的调控机制。[方法]体外培养LX-2,使用不同浓度的奥曲肽(10^-3、10^-4、10^-5、10^-6mmol/L)作用于LX-2 24 h后,采用MTT法和TUNEL荧光法分别检测LX-2的增殖和凋亡情况,采用免疫细胞化学法检测STAT3、SOCS3蛋白水平的表达,采用RT-PCR法检测STAT3和SOCS3 mRNA水平的表达。[结果]奥曲肽可以降低LX-2的增殖速度,并促进其凋亡;同时,奥曲肽能够下调STAT3、SOCS3在蛋白及mRNA水平的表达。[结论]奥曲肽可以通过抑制LX-2增殖、促进其凋亡从而延缓肝纤维化进展,并且该作用可能是通过抑制STAT3、SOCS3的表达实现的。
- 奥曲肽 /
- 人肝星状细胞 /
- 增殖 /
- 凋亡 /
- STAT3 /
- SOCS3
Abstract: [Objective]To study the effect of octreotide on proliferation,apoptosis and the expression level of STAT3 and SOCS3 in hepatic stellate cells LX-2,further to discuss the possible regulation mechanism of octreotide on human hepatic fibrosis process.[Methods]LX-2 were cultured in vitro,and were intervened by different concentrations of octreotide(10^-3,10^-4,10^-5,10^-6mmol/L)for 24 h,proliferation and apoptosis of LX-2 were detected by MTT and TUNEL,the expression of STAT3 and SOCS3 protein were measured by immunocytochemistry,and the expression of STAT3 and SOCS3 mRNA levels were detected by RT-PCR.[Results]octreotide can reduce the proliferation of LX-2,and promote apoptosis.At the same time,octreotide can enhance the proliferation of SOCS3 and STAT3 at mRNA and protein level.[Conclusion]Octreotide may inhibit LX-2 proliferation,and promote apoptosis to delay the progress of liver fibrosis,and the effect may be through inhibition the expression of STAT3 and SOCS3.
- octreotide /
- human hepatic stellate cells /
- proliferation /
- apoptosis /
- STAT3 /
- SOCS3

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