Effects of drug-containing serum of different anti-hepatic-cirrhosis prescription on related gene expression of HSC-T6 cells
-
摘要: [目的]观察抗肝硬化不同中药组方大鼠含药血清对肝硬化HSC-T6细胞相关基因表达的影响及其效果差异。[方法]将30只SD大鼠随机分为正常对照组、秋水仙碱对照组、抗肝硬化不同中药组（1号方组、2号方组、3号方组）共5组，每组6只，HSC-T6细胞复苏后常规培养，采用半定量反转录聚合酶链反应（RT-PCR）和ELISA法观察药物对肝硬化HSC-T6细胞Ⅰ型胶原（Co1Ⅰ）、Ⅲ型胶原（Co1Ⅲ）和前凋亡蛋白（Bax）的mRNA表达的影响。[结果]与正常对照组比较，抗肝硬化不同中药1号方和2号方对Co1Ⅰ、Co1Ⅲ、Bax mRNA的表达差异均有统计学意义（P<0.05，P<0.01），而3号方除对Bax mRNA的表达与正常对照组比较差异有统计学意义（P<0.05），而其对Co1Ⅰ、Co1Ⅲ mRNA的表达与正常对照组比较，差异无统计学意义。[结论]抗肝硬化不同中药组方大鼠含药血清对肝硬化HSC-T6细胞相关基因表达均有不同程度的抑制作用。Abstract: [Objective] To investigate influences and effects of drug-containing serum of different anti-hepatic-cirrhosis prescription on related gene expression of HSC-T6 cells.[Methods] Thirty SD rats were randomly divided into five groups:control group, colchicine group and three different prescription trial groups(Group 1, Group 2 and Group 3).After the resuscitation and culture of HSC-T6 cells, the influences of different drugs on mRNA expression of Collagen Type I, Collagen Type Ⅲ and apoptosis protein Bax in HSC-T6 cells were detected by semi-quantitative reverse-transcriptase polymerase chain reaction(RT-PCR) and ELISA analysis.[Results] As compared with the control group, mRNA expression of Collagen Type I, Collagen Type Ⅲ and apoptosis protein Bax showed significant differences in Group 1 and Group 2(P<0.05;P<0.01).As compared with the normal control, Group 3 had significant effect on mRNA expression of apoptosis protein Bax with no significant effect on mRNA expression of Collagen Type Ⅰ(P<0.05).[Conclusion] Drug-containing serum of different anti-hepatic-cirrhosis prescription had varying inhibitory effects on related gene expression of HSC-T6 cells.
-
-
[1] 萨姆布鲁克, 黄培堂.分子克隆实验指南[M].北京:科学出版社, 2005:1-693.
[2] 刘成海, 刘平, 胡义扬, 等.中医药抗肝纤维化临床与基础研究进展[J].世界科学技术-中医药现代化, 2007, 9(2):112-119.
[3] 胡爱荣, 丁一生, 程明亮.丹芍化胶囊对肝纤维化大鼠肝星状细胞增殖、话化及凋亡的影响[J].中华肝脏病杂志, 2006, 14(2):141-143.
[4] 陈尉华, 陆伦根, 曾民德, 等.异甘草酸镁对大鼠肝星状细胞增殖和氧应激的影响[J].中华肝脏病杂志, 2006, 14(6):426-430.
[5] 蔡锐, 伍参荣, 胡海平, 等.桃红四物汤加丹参对肝星状细胞增殖和凋亡影响[J].湖南中医学院学报, 2005, 25(6):22-24.
[6] RIPPE R A.Life or death:the fate of the hepatic stellate cell followinghepatic inhury[J].Hepatology, 1998, 27(5):1447-1448.
[7] MURPHY F R, ISSA R, ZHOU X, et al.Inhibition of apoptosis of activated hepatic stellaed cells by tissue inhibitor of metalloproteinase-1 is mediated via dffects on matrix metalloproteinase inhibition:implications for of liver fibrosis[J].J Biol Chem, 2002, 277:11069-11076.
[8] IREDALE J P, BENYON R C, PICKERING J, et al.Mechanisms of spontaneous resolution of rat liver fibrosis[J].J Clin Invest, 1998, 102:538-549.
-
计量
- 文章访问数: 64
- PDF下载数: 94
- 施引文献: 0
下载: