六味地黄汤对非酒精性脂肪性肝病大鼠SREBP-1c和LXR-α基因表达的影响

黄妙珍; 傅珍春; 谢军军; 孙丽伟

doi:10.3969/j.issn.1671-038X.2012.05.007

六味地黄汤对非酒精性脂肪性肝病大鼠SREBP-1c和LXR-α基因表达的影响

- 1. 杭州师范大学临床医学院中医学教研室, 浙江杭州 310036;
- 2. 杭州师范大学临床医学院内科实验室, 浙江杭州 310036;
- 3. 杭州师范大学附属医院消化科, 浙江杭州 310015
基金项目:
杭州市科技局资助项目（No：20090833B25）

详细信息

作者简介:
黄妙珍,教授,主任医师;主要从事脂肪肝的基础研究

通讯作者: 黄妙珍,E-mail:hmzhope@163.com

中图分类号: R575.5

收稿日期: 2012-03-20

Effect of Liuweidihuang decoction on sterol regulatory element binding protein -1c and liver X receptor α in the nonalcoholic fatty liver disease of rats

- 1. Traditional Chinese Medicine Teaching and Research Room, Clinical Medical School HangzhouUniversity, Hangzhou 310036, China;
- 2. Medical Laboratory, Clinical Medical School, Hangzhou Normal University, Hangzhou 310036, China;
- 3. Digestive Department, Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, China

More Information

Corresponding author: HUANG Miao-zhen, E-mail: hmzhope@163.com

Received Date: 20 March 2012

摘要

摘要: [目的]探讨六味地黄汤对非酒精性脂肪性肝病大鼠固醇调节元件结合蛋白（SREBP-1c）和肝X受体（LXR-α）的影响。[方法]选择雄性SD大鼠，随机分3组：正常组，六味地黄汤组（治疗组）和模型组。正常组喂养普通饲料，模型组和治疗组喂养高脂饲料建立非酒精性脂肪性肝病模型，治疗组并用六味地黄汤干预治疗；荧光定量RT-PCR 检测各组大鼠SREBP-1c 和LXR-α的基因表达。[结果]模型组大鼠SREBP-1cmRNA和LXR-αmRNA的相对表达均高于正常组（分别为t=2.361，P<0.05和t=3.118，P<0.01）；与模型组相比，治疗组能显著降低SREBP-1cmRNA和LXR-αmRNA的表达（分别为t=3.816，P<0.01和t=3.36，P<0.01）。[结论]六味地黄汤能降低SREBP-1c和LXR-α的基因表达，改善脂质代谢。
- 非酒精性脂肪性肝病 /
- 六味地黄汤 /
- 固醇调节元件结合蛋白-1c /
- 肝X受体-α
Abstract: [Objective] To explore the effect of Liuweidihuang decoction on sterol regulatory element binding protein -1c(SREBP-1c)and liver X receptor α(LXR-α)in the nonalcoholic fatty liver disease(NAFLD)of rats.[Methods] Male Sprague-Dawley rats were randomly divided into model group,treatment group,normal group.To produce a fatty liver model,a high-fat diet was given for ten weeks.The normal group rats were fed normal diet.The treatment group was given Liuweidihuang decoction while model group and normal group were given distilled water for six weeks.The expressions of SREBP-1cmRNA and LXR-αmRNA in rats liver were measured by fluorescent quantitation reverse transcription-polymerase chain raction.[Results] The expression of SREBP-1cmRNA and LXR-αmRNA in model group was higher than the normal group(t=2.361,P<0.05 and t=3.118,P<0.01 respectively).The expression of SREBP-1cmRNA and LXR-αmRNA in treatment group was lower than that of the model group(t=3.816,P<0.01;t=3.36,P<0.01 respectively).[Conclusion] Liuweidihuang decoction could reduce the expression of SREBP-1cmRNA and LXR-αmRNA in the nonalcoholic fatty liver disease of rats.
- nonalcoholic fatty liver disease /
- Liuweidihuang decoction /
- sterol regulatory element binding protein-1c /
- liver X receptor α

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