乙型肝炎患者乙肝两对半模式和乙肝病毒DNA定量相关性研究

方菊; 李欢; 綦鹏; 陈开俏

doi:10.3969/j.issn.1671-038X.2017.07.13

乙型肝炎患者乙肝两对半模式和乙肝病毒DNA定量相关性研究

- 1. 海南省三亚市妇幼保健院检验科, 海南三亚 572000;
- 2. 海南省三亚市人民医院检验科, 海南三亚 572000;
- 3. 海南省三亚市人民医院内科, 海南三亚 572000

详细信息

作者简介:
方菊,女,本科,研究方向:乙肝DNA病毒检测,疑难细菌鉴定及药敏方面

通讯作者: 李欢,E-mail:xydd2708@163.com

中图分类号: R575.1

收稿日期: 2016-12-10

Study on the quantitative correlation between hepatitis B virus DNA and hepatitis B virus in patients with hepatitis B

- 1. Department of Laboratory, Maternal and Child Health Hospital of Sanya; Hainan 572000 China;
- 2. Department of Laboratory, the People's Hospital of Sanya City, Hainan 572000 China;
- 3. Department of Internal Medicine, the People's Hospital of Sanya City, Hainan 572000 China

More Information

Corresponding author: LI Huan, E-mail: xydd2708@163.com

Received Date: 10 December 2016

摘要

摘要: [目的]探讨乙肝病毒感染患者中乙肝两对半模式和乙肝病毒DNA定量相关性。[方法]以在三亚市人民医院就诊的乙型肝炎患者500例为研究对象，分别采用酶联免疫吸附法及荧光定量PCR法测定其乙肝两对半及乙肝病毒DNA （HBV-DNA）定量，观察两者之间的相关性。[结果]共纳入乙型肝炎患者500例，其中大三阳患者HBV-DNA定量对数值显著高于其他组（P<0.05；e抗原定量与HBV-DNA对数定量相关分析显示：e抗原阳性患者中，e抗原定量值与HBV-DNA对数定量呈高度线性相关（r=0.81）；e抗原阴性患者e抗原定量值与HBV-DNA对数定量呈中度线性相关（r=0.52）。[结论]乙型肝炎患者中乙肝两对半模式与HBV-DNA定量值存在明显相关性，两者联合检测可准确反映乙型肝炎患者体内乙肝病毒复制情况，值得临床借鉴参考。
- 乙型肝炎 /
- 两对半 /
- 乙肝病毒 /
- DNA定量
Abstract: [Objective] To investigate the correlation of "two and a half pairs of hepatitis B" models and HBV DNA quantitative in HBV infected patients.[Methods] 500 patients with hepatitis B in our hospital, as the research object, and the determination of the two semi hepatitis B and hepatitis B virus DNA(HBV-DNA) were assayed by enzyme linked immunosorbent assay and fluorescence quantitative PCR quantitative.The correlation was observed between the two modes.[Results] The study included 500 patients with hepatitis B, among them whose surface antigen, e antigen and core antibody positive, HBV-DNA numerical quantification were significantly higher than that in other groups(P<0.05);e antigen quantitative correlation with HBV-DNA log quantitative analysis showed that in e antigen positive patients, the quantitative value of e antigen was high linear correlated with the logarithm of HBV-DNA(r=0.81);In e antigen negative patients, the quantitative value of e antigen was moderately linear correlation with the logarithm of HBV-DNA(r=0.52).[Conclusion] There is a significant correlation between hepatitis B two-pair model and HBV-DNA quantification in patients with hepatitis B, and both joint detection can accurately reflect the hepatitis B virus replication in vivo, it is worthy for clinical reference.
- Hepatitis B /
- Two pairs of half /
- Hepatitis B virus /
- DNA quantitative

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参考文献(11)

[1]	曾雅莉, 马清峰, 熊微, 等.不同病期慢性HBV感染者肝功能、HBV-DNA与HBV-M模式的关系[J].国际检验医学杂志, 2015, 9(4):433-435.
[2]	曾繁钰, 蒋冰, 谭璐.Pre S1抗原与HBV血清学标志物和HBV DNA的相关性及临床意义[J].武汉大学学报(医学版), 2014, 35(1):85-88.
[3]	杨沛华, 谢永忠, 夏金荣, 等.非免疫清除期乙型肝炎患者行肾结石手术后HBV-LP、HBV-M及HBV-DNA变化研究[J].山东大学学报(医学版), 2014, 52(1):104-105.
[4]	中华医学会肝病学分会, 中华医学会感染病学分会.慢性乙型肝炎防治指南(2015年更新版)[J].临床肝胆病杂志, 2015, 31(12):1941-1960.
[5]	叶远青, 罗文沈, 黄华, 等.乙型肝炎病毒preS1与乙肝两对半和HBV-DNA联合检测的临床价值分析[J].中国医药科学, 2015, 5(17):146-149.
[6]	申丽娟, 郭薇, 高平, 等.p38丝裂原活化蛋白激酶和乙型肝炎病毒X蛋白在人肝癌发生中对细胞增殖和凋亡的作用[J].中华肝脏病杂志, 2012, 20(3):227-230.
[7]	占国清, 谭华炳, 李芳, 等.HBeAg阴性慢性乙肝患者血清前S1抗原与HBV DNA的关系[J].胃肠病学和肝病学杂志, 2011, 20(9):842-844.
[8]	陈小颖, 龙璐, 李琼, 等.基于Pre-NAT全自动核酸提取平台的高敏HBV DNA检测性能评价[J].中华检验医学杂志, 2015, 8(10):696-700.
[9]	黄之品, 杨伟, 李增彩, 等.216例乙肝患者HBeAg、HBV DNA、PreS1-Ag相关性的临床分析[J].辽宁医学院学报, 2014, 25(3):42-43.
[10]	谭斌, 杨文才, 李嘉, 等.HBV-DNA水平与乙型肝炎两对半模式的相关性[J].中国老年学杂志, 2015, 35(10):2749-2750.
[11]	陈立章, 薛静, 刘立亚, 等.血清H BV-D N A定量检测与凝血指标对乙肝患者病情诊断的研究[J].中国现代医学杂志, 2013, 23(30):31-36.