Characteristics of salivary microbiota in patients with ulcerative colitis with thin white fur and yellow greasy fur
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摘要: 目的:研究溃疡性结肠炎(UC)薄白苔患者、黄腻苔患者和健康者的唾液菌群特征,以探讨UC患者舌苔与唾液菌群之间的关系及其分布差异。方法:选取符合纳入标准的门诊及住院UC薄白苔患者35例,UC黄腻苔患者35例,健康者(薄白苔)35例,收集其唾液标本。采用16S rDNA基因测序分析技术和生物信息学方法研究UC不同舌苔患者唾液菌群的多样性和丰度。结果:UC薄白苔组、UC黄腻苔组和健康对照组唾液菌群物种丰富度和多样性差异无统计学意义。健康对照组唾液菌群数量最多,UC薄白苔组唾液菌群数量其次,UC黄腻苔组最少。与UC薄白苔组比较,UC黄腻苔组唾液菌群在门水平放线菌门明显增多,在属水平放线菌属、丛毛单胞菌属等增多,罗氏菌属等减少。结论:UC薄白苔患者和UC黄腻苔患者在唾液菌群组成和细菌丰度上差异有统计学意义,黄腻苔患者唾液中存在有益菌的减少和促炎菌的增多,猜测UC黄腻苔患者唾液菌群失调更为严重。UC患者唾液菌群失调类型和功能差异,为探索舌苔形成提供了微生态学依据。Abstract: Objective: To observe the characteristics of salivary microbiota in healthy persons, Ulcerative colitis(UC) patients with thin white fur(UC with TWF) and yellow greasy fur(UC with YGF), in order to explore the relationship between tongue fur and salivary microbiota in UC patients and their distribution differences.Methods: Thirty-five patients with UC with TWF, 35 patients with UC with YGF and 35 healthy persons(thin white fur) who met the inclusion criteria were selected and saliva specimens were collected. The diversity and abundance of salivary microbiota in UC patients were studied by 16 S rDNA gene sequencing and bioinformatics.Results: There was no significant difference in the species richness and diversity of salivary microbiota among UC with TWF group、UC with YGF group and healthy control group. The number of salivary microbiota in healthy people was more than that in UC patients; the number of salivary microbiota in UC with TWF was more than that in UC with YGF. At the phylum level: Compared with the UC with TWF group, Actinobacteria in the salivary microbiota of the UC with YGF group increased significantly(P<0.05). At the genus level: Compared with the UC with TWF group, Actinomyces, Comamonas, Alloscardovia in the salivary microbiota of the UC with YGF group increased significantly(P<0.05), while Roseburia and romboutsia decreased significantly(P<0.05).Conclusion: There are differences in salivary microbiota composition and bacterial abundance between UC with TWF group and UC with YGF group. Beneficial bacteria in saliva decreases and pathogenic bacteria increases among UC with YGF group. It is speculated that UC with YGF patients have more serious salivary microbiota imbalance. The different types and degrees of salivary microbiota imbalance in UC patients provide microecological value for exploring the formation of tongue coating.
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Key words:
- ulcerative colitis /
- saliva microbiota /
- Tongue coating /
- thin white fur /
- yellow greasy fur /
- 16S rDNA
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