西黄丸含药血清通过P糖蛋白途径逆转肝癌细胞多药耐药的体外研究

李桂芝; 戴一; 黄仁宝; 童铸廷; 姚梦群; 仲飞

doi:10.3969/j.issn.1671-038X.2016.02.01

西黄丸含药血清通过P糖蛋白途径逆转肝癌细胞多药耐药的体外研究

- 1. 武警安徽省总队医院肿瘤科, 安徽合肥 230061;
- 2. 安徽新华学院药学院, 安徽合肥 230088;
- 3. 安徽中医药大学第一附属医院肿瘤科, 安徽合肥 230031;
- 4. 安徽医科大学第一附属医院肿瘤科, 安徽合肥 230022
基金项目:
安徽高校省级自然科学研究重点项目（No：KJ2012A163）

详细信息

通讯作者: 仲飞,E-mail:zhong_fei@126.com

中图分类号: R735.7

收稿日期: 2015-08-10

In vitro study on the reversal effect of serum containing Xihuang pill on the multidrug-resistance of hepatic cancer cells via P-glytoprotein pathway

- 1. Department of Oncology, Hospital of Armed Police of Anhui Province, Hefei 230061, China;
- 2. Pharmacy School, Anhui Xinhua College, Hefei 230088, China;
- 3. Department of Oncology, First Affiliated Hospital, Anhui University of Chinese Medicine, Hefei 230031, China;
- 4. Department of Oncology, the First Affiliated Hospital, Anhui Medical University, Hefei 230022, China

More Information

Corresponding author: ZHONG Fei, E-mail: zhong_fei@126.com

Received Date: 10 August 2015

摘要

摘要: [目的]探讨西黄丸含药血清对多药耐药人肝癌细胞株BEL-7404/Adr细胞和HepG2/Adr细胞耐阿霉素作用的影响及机理。[方法]制备西黄丸含药血清；BEL-7404/Adr细胞和HepG2/Adr细胞分别用西黄丸含药血清、阿霉素及二者联合处理；MTT法检测细胞增殖；Annexin V-FITC/PI双染色流式细胞术检测细胞凋亡；荧光酶标仪测定细胞内阿霉素的荧光强度；Western-blot法检测细胞P-糖蛋白（P-gp）的表达。[结果]西黄丸含药血清联合不同浓度阿霉素处理24 h后，BEL-7404/Adr细胞和HepG2/Adr细胞增殖抑制率和凋亡率高于单用同浓度阿霉素组，比较差异有统计学意义（P<0.05）；西黄丸含药血清联合不同浓度阿霉素处理12 h后，BEL-7404/Adr细胞和HepG2/Adr细胞内阿霉素的荧光强度高于单用同浓度阿霉素组，比较差异有统计学意义（P<0.05）；西黄丸含药血清处理12 h后，BEL-7404/Adr细胞和HepG2/Adr细胞P-gp蛋白表达水平降低，较对照组差异有统计学意义（P<0.05）。[结论]西黄丸含药血清显著逆转BEL-7404/Adr细胞和HepG2/Adr细胞的耐阿霉素作用，其机制可能与西黄丸含药血清下调细胞多药耐药蛋白P-gp表达相关。
- 西黄丸 /
- 多药耐药 /
- 肝细胞癌 /
- P糖蛋白
Abstract: [Objective] To investigate the reversal effects and mechanisms of serum containing Xihuang pill on adriamycin resistance of the multidrug-resistant hepatic cancer cells including the human hepatocellular carcinoma BEL-7404/Adr cell and HepG2/Adr cell.[Methods] Serum containing Xihuang pill was prepared.BEL-7404/Adr cell and HepG2/Adr cell were treated with serum containing Xihuang pill, adriamycin, or their combinations.The proliferation and apoptosis of cells were analyzed by MTT assay and flow cytometry(AnnexinV-FITC/PI double staining assay).Intracellular accumulation of adriamycin was measured by the detection of fluorescence intensity of cell lysates using microplate reader.The protein expressions of P-gp was detected by Western blot.[Results] The growth inhibition rate and apoptosis rate of the BEL-7404/Adr cell and HepG2/Adr cell treated with the combination of serum containing Xihuang pill and adriamycin were higher compared with the cells treated with adriamycin alone(P<0.05).Intracellular accumulation of adriamycin was significantly enhanced and the protein expression of P-gp decreased by the treatment of serum containing Xihuang pill(P<0.05).[Conclusion] Serum containing Xihuang pill reverses the multidrug-resistance of the human hepatocellular carcinoma BEL-7404/Adr cell and HepG2/Adr cell, and the P-glytoprotein pathway may be involved in the effect.
- Xihuang pill /
- Multidrug-resistance /
- hepatocellular carcinoma /
- P-glytoprotein

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