Abstract: [Objective] To study the significance of doctors-nurses integration training on improving comprehensive ability of laparoscopy operation room nurses.[Methods] Twelve laparoscopy nurses(integrated training group)were trained by doctors-nurses integrated training. The contents of doctors-nurses integrated training included specialized knowledge,practice skill,operation instruments preparation and cooperation of doctors and nurses during operation. Another 12 laparoscopy nurses (control group)were trained by traditional nursing training. The comprehensive ability of integrated training group was compared with that of the traditional nursing training.[Results] The excellent and good rate of professional theory achievements was 66.7%(8/12)in doctors-nurses integrated training group,and it was 58.3%(7/12) in control group. There was no significant difference between the two groups. The excellent and good rate of professional practice skills achievements in doctors-nurses integrated training group was 91.7%(11/12), which was significantly higher than control group 41.7%(5/12) (P＜0.05). The excellent and good rate of operation instruments preparation in doctors-nurses integrated training group was 87.3%(117/134), obviously higher than control group 73.5%(75/102) (P＜0.05).The doctors satisfactory rate of doctors-nurses integrated training group was 89.6%(120/134), which was higher than control group 76.5%(78/102) (P＜0.05).[Conclusion] Doctors-nurses integrated training is contributive to improving the comprehensive ability of laparoscopy operation nurses. It is a recommended way of laparoscopy nurses on-the-job training.