脾虚四号方干预大鼠腹泻模型后结肠黏膜微观结构的变化

康楠; 王凤云; 陈婷; 王晓鸽; 朱恩林; 唐旭东

doi:10.3969/j.issn.1671-038X.2015.01.01

脾虚四号方干预大鼠腹泻模型后结肠黏膜微观结构的变化

- 1. 北京中医药大学, 北京 100029;
- 2. 中国中医科学院西苑医院脾胃病科, 北京 100091
基金项目:
2013年国家重点基础研究发展计划（973计划）项目（No：2013CB531703）

详细信息

作者简介:
康楠,北京中医药大学2012级博士

通讯作者: 唐旭东,E-mail:txdly@sina.com.cn

中图分类号: R574.4

收稿日期: 2014-08-25

Microstructure changes of the colon mucosa tissue in diarrhea rats after treating by Pixu Ⅳ Recipe

- 1. Beijing University of Chinese Medicine, Beijing 100029, China;
- 2. Department of Gastroenterology, Xiyuan Hospital of China Academy of Chinese Medical Science, Beijing 100091, China

More Information

Corresponding author: TANG Xu-dong, E-mail: txdly@sina.com.cn

Received Date: 25 August 2014

摘要

摘要: [目的]从微观结构的角度，观察脾虚四号方干预大鼠腹泻模型后，大鼠结肠黏膜的变化。[方法]采用水环境小平台站立法复合高乳糖饲料喂养的方法复制腹泻模型，通过透射电镜技术观察中药复方对大鼠腹泻模型结肠黏膜细胞微观结构的影响。[结果]模型组和正常组比较，模型组结肠上皮细胞损伤明显：微绒毛形态不规则且部分缺失；细胞膜破坏，出现裸核；线粒体内脊模糊不清、肿胀、电子密度改变；杯状细胞分泌异常活跃；细胞连接的缝隙连接明显增宽。中药干预组肠黏膜细胞损伤较轻，但仍存在微绒毛形态不规整，长短不齐，线粒体电子密度增高、形态不规则，杯状细胞分泌活跃等情况。另外，中药干预组结肠黏膜细胞出现异常隆起小体。[结论]小平台站立和高乳糖饲料喂养的方法造成的大鼠腹泻模型，其结肠黏膜细胞会出现微绒毛形态改变等微观结构的变化，而中药脾虚四号方干预可以缓解其电镜下黏膜损伤。
- 脾虚四号方 /
- 腹泻 /
- 小平台站立法 /
- 高乳糖饲料 /
- 透射电镜 /
- 结肠黏膜
Abstract: [Objective] To study the microstructure changes of the colon mucosa tissue in diarrhea rats after treating by Pixu Ⅳ Recipe.[Methods] Three-week-old male rats were fed with a high-fat-lactose diet, and were forced to stand on the multiple platform which was full of water around, in order to make the rats suffer from functional diarrhea. Through the transmission electron microscopy the effect of Chinese herbal compound prescription on the colon mucosal microstructure changes were observed.[Results] Compared to the normal group, the rats in model group had serious damage in their colonic mucosa:the shape of microvilli was irregular and even lost parts of the colonic mucosa;the cell membrane damaged;the naked nucleus appeared;the mitochondria swelled, the cristae shape was not clear and the electron density changed;the goblet cells were much more active;the gap junctions widened. All the above phenomena improved in the traditional Chinese medicine therapy group, but still existed. In addition, abnormal uplift corpuscle appeared in the distal colonic mucosa of the rats in the treatment group.[Conclusion] Pixu Ⅳ Recipe can relieve the microstructure changes of the colon mucosa tissue in the diarrhea rats, which are induced by the high-fat-lactose diet and modified multiple platform method.
- Pixu Ⅳ Recipe /
- diarrhea /
- modified multiple platform method /
- high fat lactose diet /
- transmission electron microscopy /
- colon mucosa

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