Abstract: [Objective] To investigate the clinical significance of determination of D two dimer in colon cancer.[Methods] Sixty-two patients with colon cancer in our hospital were selected as the observation group,while the healthy people at the same period in the medical examination center were selected as the control. The positive rate of D two dimer in two groups,value of D two dimer in patients with colon cancer metastasis and without metastasis, patients before and after receiving chemotherapy was determined. Relationship between the D two dimer change and the symptoms’ improvement was analyzed. Relationship between clinical stage and D two dimer were investigated.[Results] The positive rate of D two dimer in the observation group was 46.77%,while it was 0% in the control group (P<0.05).The concentration of D two dimer in the patients with no metastasis,lymph node metastasis,distant organ metastasis were (0.23± 0.09) mg/L,(1.51± 1.45) mg/L,and (2.23± 1.29) mg/L,respectively(P<0.05),which indicated that the value of D two dimer aggravated with colorectal cancer metastasis.The concentration of D two dimer in stage II,III,IV of colon cancer was (0.27±0.08)mg/L,(1.16±0.36)mg/L,and(2.01±0.82)mg/L,respectively(P<0.05), which suggested the later colon cancer staging,the higher concentration of the two D dimer.After chemotherapy,the value of two D dimer in patients with colon cancer (CR+PR) decreased to(0.43±0.21)mg/L(P<0.05), and in SD patients,it decreased to (0.08±0.09)mg/L (P>0.05),in PD patients,it increased to (0.92±0.34)mg/L(P<0.05).[Conclusion] D two dimer shows close relationship with colon cancer incidence,metastasis,clinical staging and prognosis of diseases.