-
摘要: [目的]研究疏肝健脾、化痰活血方对体外非酒精性脂肪性肝病（NAFLD）模型肝细胞凋亡率及其相关基因的影响。[方法]软脂酸诱导幼鼠原代培养肝细胞复制NAFLD细胞模型，并用疏肝健脾、化痰活血方药含药血清干预，流式细胞仪检测肝细胞凋亡率，Western blot法检测凋亡相关基因bcl-2、bax和caspase-3的表达。[结果]疏肝健脾、化痰活血方能明显降低NAFLD模型体外肝细胞凋亡率，并升高Bcl-2蛋白表达水平，降低Bax和Caspase-3表达。[结论]疏肝健脾、化痰活血方能有效降低体外NAFLD模型肝细胞凋亡率，其机制可能与调节Bcl-2、Bax和Caspase-3蛋白表达有关。
-
-
[1] SANYAL A J.American gastroenterological association[J].Gastroenterology,2002,123:1705-1725.
[2] WIECKOWSKA A, ZEIN N N, YERIAN L M,et al. In vivo assessment of liver cell apoptosis as a novel biomarker of disease severity in nonalcoholic fatty liver disease[J]. Hepatology,2006,44:27-33.
[3] FELDSTEIN A E, CANBAY A, ANGULO P, et al. Hepatocyte apoptosis and fas expression are prominent features of human nonalcoholic steatohepatitis[J]. Gastroenterology.2003,125:437-443.
[4] 曾兰,盛国光,王平,等.疏肝健脾、化痰活血方对NAFLD体外细胞模型线粒体脂质过氧化的影响[J].湖北中医杂志,2011,33(4):7-8.
[5] 曾兰,盛国光.疏肝活血化痰放对NAFLD体外细胞模型线粒体能量代谢的影响[J].山西中医,2011,27(3):38-40.
[6] 胡义扬,张慧,陈少东,等.一种用于脂肪肝脂毒性药理研究的体外模型[J].中华肝脏病杂志,2008,16(2):121-124.
[7] KERR J F,WYLLIE A H,CURRIE A R.Apoptosis:a basic biological phenomenon with wide-ranging implications in tissue kinetics[J].Br J Cancer,1972,26:239-257.
[8] FERNANDES-ALNEMRI T,LITWACK G,ALNEMRI E S.CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme[J]. J Biol Chem.1994,269:30761-30764.
[9] JAESCHKE H,FISHER M A,LAWSON J A,et al.Activation of caspase 3(CPP32)-like proteases is essential for TNF-alpha-induced hepatic parenchymal cell apoptosis and neutrophil-mediated necrosis in a murine endotoxin shock model[J]. J Immunol,1998,160:3480-3486.
-
计量
- 文章访问数: 81
- PDF下载数: 206
- 施引文献: 0
下载: